{"id":"W2996715978","doi":"10.1021/acssynbio.9b00456","title":"A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis","year":2019,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":40,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Japan Society for the Promotion of Science; Natural Sciences and Engineering Research Council of Canada; Genome Canada; National Science Foundation","keywords":"Nucleoside-diphosphate kinase; Biochemistry; Kinase; GTP'; Polyphosphate; Cell-free protein synthesis; Nucleoside triphosphate; Protein kinase A; Phosphofructokinase 2; Nucleoside; Biology; Protein biosynthesis; Chemistry; Cell biology; Enzyme; Nucleotide; Phosphate; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002581415,0.0001573342,0.0001813066,0.00004334735,0.00007068156,0.00001267316,0.0002108873,0.0001695826,0.00006170048],"category_scores_gemma":[0.0002133765,0.0001195002,0.00006168665,0.00007574487,0.000164714,0.000004029558,0.0001437264,0.00008221762,0.000008922234],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007508608,"about_ca_system_score_gemma":0.00004781904,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004618324,"about_ca_topic_score_gemma":0.00002316503,"domain_scores_codex":[0.9990716,0.00007547089,0.0002449491,0.0003323555,0.0000628966,0.0002127079],"domain_scores_gemma":[0.999227,0.00006647634,0.0001039628,0.0005031495,0.00005214567,0.00004727965],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005067652,0.00002476906,0.001784893,0.00002677802,0.00003864752,4.822763e-7,0.00002091163,0.0001443051,0.99155,0.0008895755,0.00011427,0.005354709],"study_design_scores_gemma":[0.00035908,0.0001686548,0.001418911,0.00003468722,0.00003054784,0.0000192136,0.00004403869,0.0002766852,0.9917743,0.0002623444,0.005455423,0.0001561557],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9927614,0.00249406,0.003015805,0.0004852702,0.0002497584,0.0003520918,0.00002307519,0.00001329987,0.0006052595],"genre_scores_gemma":[0.9981115,0.0002622474,0.001028282,0.00005694621,0.00007015944,0.00005839292,0.00001802179,0.0000181456,0.0003762491],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005350176,"threshold_uncertainty_score":0.4873075,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004760066890453769,"score_gpt":0.2350407151799278,"score_spread":0.230280648289474,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}