{"id":"W2998455966","doi":"10.1016/j.omtn.2019.12.021","title":"A Universal Surrogate Reporter for Efficient Enrichment of CRISPR/Cas9-Mediated Homology-Directed Repair in Mammalian Cells","year":2019,"lang":"en","type":"article","venue":"Molecular Therapy — Nucleic Acids","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"National Major Science and Technology Projects of China; National Natural Science Foundation of China","keywords":"CRISPR; Cas9; Genome editing; Homology directed repair; Computational biology; Biology; Trans-activating crRNA; DNA; Genetics; DNA repair; Gene; Nucleotide excision repair","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000603431,0.0004225006,0.0008066641,0.0003165513,0.0002116449,0.0004857085,0.0005695563,0.0006110831,0.0007995192],"category_scores_gemma":[0.0004570429,0.0002621988,0.0004217699,0.0002790332,0.0002768055,0.0002905396,0.0006479921,0.0008590365,0.0008088786],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003871095,"about_ca_system_score_gemma":0.0003083213,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003041119,"about_ca_topic_score_gemma":0.0004752997,"domain_scores_codex":[0.9992343,0.0001196327,0.00009501581,0.0002110983,0.0002720861,0.0000678248],"domain_scores_gemma":[0.99977,0.00003425103,0.00005856786,0.00004624253,0.00004756949,0.00004343114],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002123526,0.000008596798,0.00006961895,0.00004637308,0.000003707793,0.00003843038,0.00001421451,0.00009915839,0.9977385,0.0002149117,0.0000688776,0.001676366],"study_design_scores_gemma":[0.000004183409,0.00006530585,0.000273223,0.000007303213,0.000007003306,0.0002149464,0.000008393554,0.001467992,0.992784,0.00004816344,0.005112724,0.00000683825],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5616313,0.003597617,0.4239819,0.0003427907,0.0002395417,0.0003976048,0.002366134,0.002970234,0.004472817],"genre_scores_gemma":[0.8231733,0.002210055,0.1609222,0.0002294451,0.00003534891,0.0003774205,0.004395896,0.0003877329,0.008268585],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008066641,"threshold_uncertainty_score":0.003191292,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005032995021164857,"score_gpt":0.2487061654001357,"score_spread":0.2436731703789708,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}