{"id":"W3001612416","doi":"10.1016/j.heliyon.2020.e03246","title":"Improving sensitivity of single tube nested PCR to detect fastidious microorganisms","year":2020,"lang":"en","type":"article","venue":"Heliyon","topic":"Mycobacterium research and diagnosis","field":"Medicine","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministry of Agriculture - Saskatchewan","keywords":"Fastidious organism; Nested polymerase chain reaction; Taq polymerase; TaqMan; Biology; Polymerase chain reaction; Microorganism; Primer (cosmetics); Thermus aquaticus; Microbiology; Molecular biology; Bacteria; Genetics; Chemistry; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001827188,0.0001256708,0.0003063907,0.0000741164,0.00003590663,0.0000192876,0.00005397493,0.00007145946,0.0001268266],"category_scores_gemma":[0.001676238,0.0001148977,0.00007919697,0.0003289896,0.000029735,0.00004973289,0.0001039676,0.0001441885,0.0002051531],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005187762,"about_ca_system_score_gemma":0.00009844608,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000142469,"about_ca_topic_score_gemma":0.00005120908,"domain_scores_codex":[0.9988664,0.00008358263,0.0002008369,0.0002675722,0.0002629825,0.0003185924],"domain_scores_gemma":[0.9989348,0.000168077,0.00004570904,0.0002121529,0.000155523,0.0004837239],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002993439,0.00006080809,0.005586236,0.0007159908,0.00001781551,0.0002201894,0.000275173,0.000002193135,0.9794549,0.000001598502,0.0003156115,0.01305009],"study_design_scores_gemma":[0.0006308174,0.001305919,0.02870725,0.0002192464,0.00003547266,0.0000640155,0.00007777725,0.00007279163,0.9678428,0.000001232753,0.0009337328,0.0001089474],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9924166,0.0003194187,0.003958506,0.002473883,0.00007128828,0.0003856982,0.00003039155,0.00008763011,0.0002566194],"genre_scores_gemma":[0.9953632,0.00004522903,0.002410978,0.001873158,0.0002179247,0.000007747598,0.00001097693,0.0000277959,0.0000430073],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02312102,"threshold_uncertainty_score":0.4685391,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02580311204544325,"score_gpt":0.2585068177197576,"score_spread":0.2327037056743144,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}