{"id":"W3002717075","doi":"10.1002/bit.27279","title":"Large‐scale expansion of feeder‐free mouse embryonic stem cells serially passaged in stirred suspension bioreactors at low inoculation densities directly from cryopreservation","year":2020,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Embryonic stem cell; Laboratory flask; Bioreactor; Cryopreservation; Cell biology; Homeobox protein NANOG; Biology; Flow cytometry; Bioprocess; Cell culture; Trypsinization; Fibroblast; Embryoid body; Stem cell; Induced pluripotent stem cell; Chemistry; Molecular biology; Embryo; Biochemistry; Botany; Genetics; Trypsin","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001476711,0.0001905187,0.0002490974,0.0001395375,0.00005741435,0.00001425467,0.000212107,0.0005725311,0.000005633104],"category_scores_gemma":[0.00004998345,0.0001909359,0.00004885575,0.0002156716,0.00008040482,0.0000157265,0.000484961,0.0001822263,0.00000283088],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003505341,"about_ca_system_score_gemma":0.00002860715,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005027642,"about_ca_topic_score_gemma":0.0002332935,"domain_scores_codex":[0.9988079,0.00005825379,0.0002725446,0.0004268639,0.0001513723,0.00028305],"domain_scores_gemma":[0.9994315,0.00002558726,0.00009046883,0.0003336289,0.00004904703,0.00006976513],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002113835,0.00002554592,0.002813175,0.00007702145,0.00003068646,0.000006863456,0.0001546314,0.0006632801,0.9957547,0.000005912796,0.000102421,0.0001543543],"study_design_scores_gemma":[0.000870011,0.0001682283,0.004035886,0.00004380195,0.00001082891,0.000001943202,0.0003138229,0.009815349,0.9842492,0.00000484063,0.0003016344,0.0001844909],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964664,0.0006432505,0.001630832,0.0007672489,0.00008968818,0.0002582784,0.00007394683,0.00006714416,0.00000320646],"genre_scores_gemma":[0.9986501,0.0005040739,0.000534649,0.00002917715,0.00004734624,0.000008233299,0.0001376237,0.00002532827,0.00006348363],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01150556,"threshold_uncertainty_score":0.7786142,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01107862930715736,"score_gpt":0.2042631707540384,"score_spread":0.193184541446881,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}