{"id":"W3002717075","doi":"10.1002/bit.27279","title":"Large‐scale expansion of feeder‐free mouse embryonic stem cells serially passaged in stirred suspension bioreactors at low inoculation densities directly from cryopreservation","year":2020,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Embryonic stem cell; Laboratory flask; Bioreactor; Cryopreservation; Cell biology; Homeobox protein NANOG; Biology; Flow cytometry; Bioprocess; Cell culture; Trypsinization; Fibroblast; Embryoid body; Stem cell; Induced pluripotent stem cell; Chemistry; Molecular biology; Embryo; Biochemistry; Botany; Genetics; Trypsin","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005339656,0.0004557151,0.000255129,0.0003345257,0.0001407106,0.0003015251,0.0003296668,0.0001727354,0.0007574651],"category_scores_gemma":[0.0002108762,0.0001415187,0.0003026429,0.0001782117,0.0001633407,0.0001726716,0.0002253895,0.0004740615,0.0004696684],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002579213,"about_ca_system_score_gemma":0.0002226302,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005061815,"about_ca_topic_score_gemma":0.0009093406,"domain_scores_codex":[0.9998494,0.00002340543,0.00002059566,0.00003338285,0.00005813855,0.0000149399],"domain_scores_gemma":[0.9998505,0.00005385436,0.00002542044,0.00002271208,0.00002732341,0.00002019043],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002357825,0.0000173414,0.00017001,0.00001252496,0.000002473166,0.00001714052,0.00001391457,0.0001761038,0.9985398,0.00004147404,0.00002465979,0.0009610109],"study_design_scores_gemma":[0.000008765935,0.0001857742,0.001318309,0.000005381001,0.00001536118,0.00003086654,0.000006624882,0.001842537,0.9956331,0.00002587745,0.0009248583,0.000002616827],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9411672,0.001169095,0.05331327,0.00009666983,0.00006208122,0.0002766671,0.0009571627,0.0004360275,0.00252198],"genre_scores_gemma":[0.9460891,0.0009153876,0.04690414,0.00004966111,0.00002990517,0.0002541663,0.001870487,0.00008616735,0.003800991],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007574651,"threshold_uncertainty_score":0.002823949,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01107862930715736,"score_gpt":0.2042631707540384,"score_spread":0.193184541446881,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}