{"id":"W3002880036","doi":"10.1016/j.enzmictec.2020.109508","title":"CRISPR/Cas9 facilitates rapid generation of constitutive forms of transcription factors in Aspergillus niger through specific on-site genomic mutations resulting in increased saccharification of plant biomass","year":2020,"lang":"en","type":"article","venue":"Enzyme and Microbial Technology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek; Consejo Nacional de Ciencia y Tecnología; Concordia University; Academy of Finland","keywords":"Aspergillus niger; Biology; CRISPR; Gene; Genetics; Cas9; genomic DNA; Genome editing; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002403235,0.0004671552,0.0002481735,0.0003226484,0.0001821636,0.0005691978,0.0004798232,0.0004189368,0.001625979],"category_scores_gemma":[0.0002091976,0.0002770601,0.0003005737,0.0001929849,0.0003452965,0.0002614058,0.0004578191,0.0009067989,0.000693245],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004521417,"about_ca_system_score_gemma":0.00029406,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001097884,"about_ca_topic_score_gemma":0.002143046,"domain_scores_codex":[0.999712,0.00003179307,0.00003807876,0.00007565215,0.0001066868,0.00003564477],"domain_scores_gemma":[0.99981,0.00003920984,0.00006780469,0.00003231585,0.00001461302,0.00003602513],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003063762,0.00001348882,0.00007766832,0.00001522636,0.000003142979,0.00004000246,0.000007345851,0.0001263357,0.9982527,0.0002594748,0.00003601651,0.001138097],"study_design_scores_gemma":[0.000005099814,0.00001816985,0.0005862411,0.000002949104,0.000005092641,0.0001013369,0.00001060433,0.001311196,0.9960003,0.00008790458,0.001865593,0.000005424139],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8923988,0.0006620853,0.09464702,0.0003377675,0.0001888576,0.0001653965,0.002004213,0.002334059,0.007261764],"genre_scores_gemma":[0.9755082,0.0002734565,0.0185673,0.0000473191,0.000006159376,0.00003364725,0.0007731316,0.0001483333,0.004642571],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001625979,"threshold_uncertainty_score":0.00543946,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02265670936856704,"score_gpt":0.2541899736255104,"score_spread":0.2315332642569434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}