{"id":"W3002880036","doi":"10.1016/j.enzmictec.2020.109508","title":"CRISPR/Cas9 facilitates rapid generation of constitutive forms of transcription factors in Aspergillus niger through specific on-site genomic mutations resulting in increased saccharification of plant biomass","year":2020,"lang":"en","type":"article","venue":"Enzyme and Microbial Technology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek; Consejo Nacional de Ciencia y Tecnología; Concordia University; Academy of Finland","keywords":"Aspergillus niger; Biology; CRISPR; Gene; Genetics; Cas9; genomic DNA; Genome editing; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00007697316,0.0001031438,0.0001958081,0.000160937,0.00001814527,0.000002523135,0.00006629351,0.0001739067,0.000003526465],"category_scores_gemma":[0.00005222873,0.0001024932,0.00003563756,0.0001878332,0.0001415133,0.000007042084,0.00002017616,0.00007827424,3.716687e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001238696,"about_ca_system_score_gemma":0.0000266697,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000118719,"about_ca_topic_score_gemma":0.0001415592,"domain_scores_codex":[0.9992288,0.00002197312,0.0003858154,0.0002179802,0.00003584308,0.0001095258],"domain_scores_gemma":[0.9997223,0.00001308897,0.0001035554,0.000108709,0.00003672145,0.00001564792],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007819766,0.000040483,0.002500637,0.00004346093,0.00001522923,8.774283e-7,0.001206225,0.0004194913,0.9950051,0.0003474672,0.00002563661,0.0003172353],"study_design_scores_gemma":[0.000821445,0.0002381475,0.00296132,0.00002501863,0.000006895619,0.000002693724,0.0006681283,0.0001482735,0.9944673,0.00003443455,0.0005363224,0.00009000147],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9926866,0.001044901,0.005593954,0.0000954029,0.00003612668,0.0002169885,0.0002964002,0.000007434026,0.00002223265],"genre_scores_gemma":[0.998554,0.0003890308,0.0006697201,0.00001231611,0.00001402703,0.000006462993,0.0003461543,0.00000663377,0.000001684982],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00586741,"threshold_uncertainty_score":0.4179551,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02265670936856704,"score_gpt":0.2541899736255104,"score_spread":0.2315332642569434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}