{"id":"W3004270985","doi":"10.1128/jb.00644-19","title":"HK97 gp74 Possesses an α-Helical Insertion in the ββα Fold That Affects Its Metal Binding, <i>cos</i> Site Digestion, and <i>In Vivo</i> Activities","year":2020,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Government of Canada","keywords":"Biology; Binding site; In vivo; Fold (higher-order function); Biochemistry; Plasma protein binding; Metal; Digestion (alchemy); Molecular biology; Genetics; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000900935,0.0002473982,0.0001735991,0.0001226265,0.0001295802,0.0002301823,0.0002468828,0.0002048937,0.001122442],"category_scores_gemma":[0.0001382069,0.00009339764,0.0001869007,0.0001550796,0.0004085726,0.0001240245,0.0001412891,0.0004173957,0.0003152466],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003203529,"about_ca_system_score_gemma":0.0001909519,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001169731,"about_ca_topic_score_gemma":0.001357374,"domain_scores_codex":[0.9999068,0.00001717088,0.00000856826,0.00001227343,0.0000363921,0.00001884092],"domain_scores_gemma":[0.9998482,0.00004318005,0.00005383061,0.00001875741,0.000009941863,0.00002600709],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009298884,0.00002258776,0.0004114001,0.00001730087,0.000009529261,0.0001305699,0.00001536948,0.00006945957,0.9980779,0.00008030011,0.00003525993,0.001037337],"study_design_scores_gemma":[0.000008444556,0.0001087888,0.007260296,0.000003961864,0.00001645615,0.0004671297,0.00006161591,0.0004669453,0.9899961,0.00003898978,0.001563031,0.000008277481],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9982022,0.00006838711,0.001069628,0.00003147924,0.000007757175,0.00000466973,0.0001008206,0.00002519223,0.0004899171],"genre_scores_gemma":[0.9976422,0.00006585816,0.0009780823,0.0000266536,0.000003920181,0.000005273551,0.0003659485,0.00003154549,0.0008805222],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001169731,"threshold_uncertainty_score":0.003754973,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0131543272635986,"score_gpt":0.2742466119532198,"score_spread":0.2610922846896211,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}