{"id":"W3005626700","doi":"10.1101/2020.02.18.953695","title":"PCR procedures to amplify GC-rich DNA sequences of <i>Mycobacterium bovis</i>","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; Canadian Food Inspection Agency","funders":"Canadian Food Inspection Agency","keywords":"GC-content; Biology; Gene; Polymerase chain reaction; Multiple displacement amplification; DNA; Genome; Recombinant DNA; Molecular biology; Genetics; Computational biology; DNA extraction","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001103164,0.001514572,0.001258594,0.001261133,0.0005859352,0.0005750407,0.001049478,0.0008251439,0.005525129],"category_scores_gemma":[0.002028256,0.001182602,0.001860705,0.0008158152,0.000697898,0.0005188333,0.0005785106,0.002436004,0.006443775],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004042232,"about_ca_system_score_gemma":0.000831898,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000540009,"about_ca_topic_score_gemma":0.001256386,"domain_scores_codex":[0.9977583,0.0004573239,0.0003350966,0.0007753918,0.0004223325,0.0002515646],"domain_scores_gemma":[0.9985654,0.0005091664,0.0003391945,0.00022634,0.0002820328,0.0000779562],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009692757,0.00007043156,0.000198556,0.0003877922,0.0000157998,0.0001380392,0.00008815347,0.0001347455,0.992538,0.0002671564,0.0002536263,0.005810618],"study_design_scores_gemma":[0.00004010428,0.0006180304,0.002095035,0.0001207965,0.0001005471,0.0005886473,0.00007316803,0.001084883,0.9749577,0.0002234859,0.02005766,0.00003992942],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2239293,0.005372327,0.7444093,0.0005142724,0.0007347381,0.006828712,0.005769999,0.004195296,0.008246073],"genre_scores_gemma":[0.2192102,0.003682476,0.732336,0.0009065621,0.0001577021,0.007183528,0.02134815,0.001440727,0.01373463],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005525129,"threshold_uncertainty_score":0.01848346,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01271973106031779,"score_gpt":0.2435408375344233,"score_spread":0.2308211064741056,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}