{"id":"W3006886971","doi":"10.1002/anie.202000621","title":"A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to <i>O</i>‐GlcNAc Transferase","year":2020,"lang":"en","type":"article","venue":"Angewandte Chemie International Edition","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Canadian Glycomics Network; Australian Research Council; National Health and Medical Research Council; Natural Sciences and Engineering Research Council of Canada","keywords":"Glycosyltransferase; Transferase; High-throughput screening; Fluorescence; Throughput; Chemistry; Computational biology; Computer science; Biochemistry; Biology; Enzyme; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009171343,0.001130847,0.0005553092,0.0004461237,0.0003295285,0.0006437196,0.0008597784,0.001314724,0.001283051],"category_scores_gemma":[0.0008382873,0.000360512,0.0005030758,0.0006679651,0.0005755704,0.0005707959,0.0006207985,0.001483256,0.0007775509],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006955194,"about_ca_system_score_gemma":0.0005179666,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008792115,"about_ca_topic_score_gemma":0.001932113,"domain_scores_codex":[0.9991441,0.0001746702,0.00004776057,0.0002248957,0.0002945272,0.0001140388],"domain_scores_gemma":[0.9996269,0.0001196887,0.00008403287,0.00004963744,0.00006751393,0.0000522343],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002976212,0.00004070033,0.0001658074,0.00008557821,0.000009468448,0.0000147738,0.0000167701,0.0001553185,0.9949142,0.0002828368,0.0002393054,0.004045498],"study_design_scores_gemma":[0.000006191899,0.0001408423,0.0004095274,0.000006596669,0.00001073811,0.00005285265,0.000007576473,0.001380642,0.9962656,0.00005885819,0.001651946,0.000008706615],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4114699,0.006774778,0.5657862,0.0007230806,0.0002825768,0.0004955699,0.0024112,0.002063845,0.009992841],"genre_scores_gemma":[0.6853383,0.00750566,0.2913544,0.000349766,0.00007077266,0.0007767362,0.002631078,0.0001494477,0.01182376],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001314724,"threshold_uncertainty_score":0.005046427,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02120615700962804,"score_gpt":0.283515635183257,"score_spread":0.262309478173629,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}