{"id":"W3007058782","doi":"10.1101/2020.02.21.959445","title":"Combining rational design and continuous evolution on minimalist proteins that target DNA","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Protein Degradation and Inhibitors","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; California HIV/AIDS Research Program","keywords":"Leucine zipper; Zipper; DNA; Rational design; Transcription factor; Computational biology; Biology; Transcription (linguistics); Enhancer; Cell biology; Genetics; Computer science; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006729541,0.0004621824,0.0004545644,0.0003542183,0.0002349438,0.0005095738,0.0005317701,0.0003050085,0.0005759367],"category_scores_gemma":[0.0007933824,0.0003006012,0.0003456559,0.0002925815,0.000434301,0.0003674111,0.0005145923,0.0006563265,0.0002149254],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007209188,"about_ca_system_score_gemma":0.0004762117,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004843481,"about_ca_topic_score_gemma":0.0007247229,"domain_scores_codex":[0.9997367,0.00004963603,0.00001974956,0.00005267814,0.00009128437,0.00004986943],"domain_scores_gemma":[0.9998148,0.00005929923,0.00004065192,0.00002208799,0.00003493307,0.00002823578],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005298175,0.0005363055,0.002059055,0.0003841155,0.00008303784,0.0003373218,0.0001908593,0.142473,0.7903777,0.02170838,0.0004275351,0.04089291],"study_design_scores_gemma":[0.0002178618,0.002454632,0.001276057,0.00004308285,0.00007370217,0.0002484558,0.000121849,0.2952982,0.6780161,0.004952822,0.01722706,0.00007024124],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8227546,0.0006997649,0.1714153,0.0002282199,0.000061031,0.0002736561,0.0001040599,0.0003811736,0.004082236],"genre_scores_gemma":[0.8896983,0.0006453957,0.1074192,0.00007122318,0.00001303818,0.0002723974,0.0002027447,0.0001083241,0.00156942],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007209188,"threshold_uncertainty_score":0.005230665,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01800875905710977,"score_gpt":0.2159225698603055,"score_spread":0.1979138108031957,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}