{"id":"W3007937893","doi":"10.1021/acschembio.9b01044","title":"Selective Fluorogenic β-Glucocerebrosidase Substrates for Convenient Analysis of Enzyme Activity in Cell and Tissue Homogenates","year":2020,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Lysosomal Storage Disorders Research","field":"Medicine","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"FP7 Ideas: European Research Council; Natural Sciences and Engineering Research Council of Canada; European Commission; Canadian Glycomics Network; Royal Society; Networks of Centres of Excellence of Canada; Canada Research Chairs; Government of Canada; Michael J. Fox Foundation for Parkinson's Research","keywords":"Glucocerebrosidase; Enzyme; Biochemistry; Biology; Cell; Mutant; Glycoside hydrolase; Gaucher's disease; Recombinant DNA; Substrate specificity; Chemistry; Gene; Disease; Medicine; Pathology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009297992,0.000123983,0.0005421426,0.0001230009,0.00001592662,0.000003909828,0.00008941306,0.0001818809,0.00004836397],"category_scores_gemma":[0.0003385597,0.0001060596,0.00009251219,0.0007192584,0.0001976142,0.00002546987,0.00006996305,0.0001740587,0.000002892477],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004555798,"about_ca_system_score_gemma":0.0000608559,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001212054,"about_ca_topic_score_gemma":0.0000231395,"domain_scores_codex":[0.9990259,0.00004723899,0.0002082111,0.000368966,0.00007594345,0.0002737667],"domain_scores_gemma":[0.9992321,0.0003303848,0.00006395682,0.0001102819,0.0001136518,0.0001496592],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006504222,0.0001749032,0.05747927,0.0001177313,0.000289296,0.000004040754,0.0002734253,0.000001241413,0.9399028,0.00002991618,0.00004418564,0.001032713],"study_design_scores_gemma":[0.001493947,0.0005097071,0.006831056,0.000004308708,0.000248652,0.000001854029,0.00006937057,0.0009831084,0.9896237,0.00006828812,0.00007222263,0.00009380614],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976425,0.0009451434,0.00007014609,0.0007598336,0.000006429643,0.0004235076,0.00007664675,0.00001501947,0.00006084375],"genre_scores_gemma":[0.9993843,0.00007335479,0.0001763529,0.0001509755,0.00002046082,0.00004847395,0.0001265465,0.00001025104,0.000009270958],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05064821,"threshold_uncertainty_score":0.4324986,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02255847537461029,"score_gpt":0.3029497630689809,"score_spread":0.2803912876943706,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}