{"id":"W3008580617","doi":"10.1002/mbo3.1001","title":"Streamlined production, purification, and characterization of recombinant extracellular polyhydroxybutyrate depolymerases","year":2020,"lang":"en","type":"article","venue":"MicrobiologyOpen","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Foundation for Innovation; Alberta Agriculture and Forestry","keywords":"Comamonas testosteroni; Pseudomonas stutzeri; Recombinant DNA; Polyhydroxybutyrate; Ralstonia; Escherichia coli; Chemistry; Heterologous expression; Polyhydroxyalkanoates; Strain (injury); lac operon; Biochemistry; Heterologous; Microbiology; Bacteria; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006258747,0.0006926285,0.0006011792,0.0003986326,0.0001713956,0.0006596579,0.0003292349,0.0004096321,0.000335815],"category_scores_gemma":[0.0007124364,0.0002290059,0.0005792718,0.0005377759,0.0002521235,0.0003991064,0.0004148097,0.0007994903,0.0007022701],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003354793,"about_ca_system_score_gemma":0.0004455766,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009715581,"about_ca_topic_score_gemma":0.001098798,"domain_scores_codex":[0.9994501,0.00008067898,0.0001060885,0.00008665453,0.0002143209,0.00006219009],"domain_scores_gemma":[0.9996581,0.00006057417,0.00008743176,0.00006679421,0.00009634827,0.00003067172],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000007863198,0.00001510188,0.0001218761,0.00004573174,0.000004357326,0.00002344135,0.000009206092,0.0001060911,0.9981286,0.00003638098,0.00001413623,0.001487211],"study_design_scores_gemma":[0.000003129188,0.0000472594,0.0009427808,0.000004764699,0.00000998286,0.00009724096,0.0000151075,0.0005190556,0.9971831,0.00002314365,0.00114957,0.000004947656],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8316462,0.002880121,0.1604411,0.0004723693,0.0001264849,0.0004150152,0.001962896,0.0004355436,0.001620296],"genre_scores_gemma":[0.8305648,0.005674461,0.152144,0.0001339369,0.00005192537,0.0003146059,0.006642819,0.0001625832,0.004310939],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009715581,"threshold_uncertainty_score":0.003310025,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02230149506850615,"score_gpt":0.2031574824492486,"score_spread":0.1808559873807425,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}