{"id":"W3008580617","doi":"10.1002/mbo3.1001","title":"Streamlined production, purification, and characterization of recombinant extracellular polyhydroxybutyrate depolymerases","year":2020,"lang":"en","type":"article","venue":"MicrobiologyOpen","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Foundation for Innovation; Alberta Agriculture and Forestry","keywords":"Comamonas testosteroni; Pseudomonas stutzeri; Recombinant DNA; Polyhydroxybutyrate; Ralstonia; Escherichia coli; Chemistry; Heterologous expression; Polyhydroxyalkanoates; Strain (injury); lac operon; Biochemistry; Heterologous; Microbiology; Bacteria; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001762299,0.0001264742,0.0002369097,0.00002981479,0.0001189812,0.00003394366,0.0001753369,0.00007421238,0.0002808857],"category_scores_gemma":[0.0000545635,0.00009720588,0.00002530654,0.00009736487,0.0001739625,0.000160777,0.00006497827,0.00004732788,0.00004732984],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005181507,"about_ca_system_score_gemma":0.00003525631,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001046199,"about_ca_topic_score_gemma":0.000005426915,"domain_scores_codex":[0.9989892,0.0001300309,0.0003382084,0.0003440322,0.00003946136,0.0001591133],"domain_scores_gemma":[0.9994942,0.00001728332,0.0001968697,0.0001787496,0.00005705728,0.00005587482],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000675572,0.00003577402,0.001234433,0.00003871144,0.000008995483,5.0338e-7,0.0003772498,1.628717e-7,0.9926423,0.0000591044,0.0001499222,0.005385314],"study_design_scores_gemma":[0.0001226477,0.00008415552,0.001237414,0.00002149323,0.00001851919,0.00001116374,0.00005486192,0.00002262555,0.9942258,0.000009739541,0.004068734,0.0001228605],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9883797,0.005738008,0.000161727,0.005127825,0.0002726267,0.0002062604,0.00004988013,0.00004399801,0.00002004277],"genre_scores_gemma":[0.998378,0.0002465101,0.0004512665,0.0001934794,0.00006398414,0.00001754304,0.00007429342,0.00001252362,0.0005623772],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009998391,"threshold_uncertainty_score":0.3963941,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02230149506850615,"score_gpt":0.2031574824492486,"score_spread":0.1808559873807425,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}