{"id":"W3011470936","doi":"10.1021/acschembio.0c00081","title":"Nonlinear Biosynthetic Assembly of Alpiniamide by a Hybrid <i>cis</i>/<i>trans</i>-AT PKS-NRPS","year":2020,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Conselho Nacional de Desenvolvimento Científico e Tecnológico; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Pesquisa do Estado de São Paulo","keywords":"Polyketide; Nonribosomal peptide; Polyketide synthase; Streptomyces; Terminator (solar); Biosynthesis; Stereochemistry; Heterologous expression; Biology; Mutagenesis; Assembly line; Biochemistry; Bacteria; Enzyme; Chemistry; Gene; Genetics; Mutation; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000122626,0.0002628854,0.000693165,0.00003728704,0.00003490542,0.000005098232,0.00025726,0.0002612161,0.0003144562],"category_scores_gemma":[0.0005801839,0.0001915615,0.0001898177,0.0002161742,0.0003374138,0.00002992387,0.0001457107,0.0003225284,0.00008862728],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003613253,"about_ca_system_score_gemma":0.0000464518,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002225159,"about_ca_topic_score_gemma":7.191483e-7,"domain_scores_codex":[0.9983216,0.00005352095,0.0004976353,0.0005928766,0.0001283445,0.0004060285],"domain_scores_gemma":[0.999009,0.0001716788,0.0001375005,0.0003155183,0.0001051444,0.0002611416],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005956329,0.0001539364,0.0001087205,0.0001182417,0.00008306028,0.00001183268,0.00003643048,2.8766e-8,0.9696286,0.00001294364,0.02562225,0.003628332],"study_design_scores_gemma":[0.0006976201,0.0003518071,0.000005682418,0.00003345358,0.00009888287,0.00006216325,0.000005863193,0.00001754818,0.8270369,0.00002082971,0.1714944,0.0001749047],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9658644,0.001135239,0.00003988588,0.03168056,0.00008932031,0.0002878328,0.0003681872,0.00008286518,0.0004517311],"genre_scores_gemma":[0.9907367,0.0003470108,0.001111467,0.006964202,0.0003270265,0.000001576351,0.0003579911,0.00002978559,0.0001242507],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1458721,"threshold_uncertainty_score":0.781165,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01334267887999408,"score_gpt":0.242246627904256,"score_spread":0.2289039490242619,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}