{"id":"W3011470936","doi":"10.1021/acschembio.0c00081","title":"Nonlinear Biosynthetic Assembly of Alpiniamide by a Hybrid <i>cis</i>/<i>trans</i>-AT PKS-NRPS","year":2020,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Conselho Nacional de Desenvolvimento Científico e Tecnológico; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Pesquisa do Estado de São Paulo","keywords":"Polyketide; Nonribosomal peptide; Polyketide synthase; Streptomyces; Terminator (solar); Biosynthesis; Stereochemistry; Heterologous expression; Biology; Mutagenesis; Assembly line; Biochemistry; Bacteria; Enzyme; Chemistry; Gene; Genetics; Mutation; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001463491,0.0003566322,0.000195776,0.0001136469,0.0001544516,0.0004081423,0.0002382966,0.0001667981,0.0007492329],"category_scores_gemma":[0.0001178001,0.0001695531,0.0002776241,0.0001247601,0.0002393347,0.0002655041,0.000369216,0.0004416112,0.0004909402],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003472917,"about_ca_system_score_gemma":0.0002160522,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003754082,"about_ca_topic_score_gemma":0.0004936075,"domain_scores_codex":[0.999895,0.00001602808,0.000009695485,0.00003321472,0.00002788054,0.00001827032],"domain_scores_gemma":[0.9998995,0.00001229362,0.00003484874,0.00001628677,0.00001076361,0.00002633418],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000470684,0.00001082364,0.0001259757,0.00001965526,0.000003586018,0.00004891977,0.00001612606,0.0002602098,0.998055,0.0004037058,0.00001647435,0.0009925035],"study_design_scores_gemma":[0.000008163809,0.0001366196,0.0002575085,0.000001886225,0.00000998542,0.0001185785,0.00001258008,0.002050921,0.9955788,0.00007256865,0.001744932,0.000007433475],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9696525,0.0002002146,0.02628126,0.00006863495,0.00003269502,0.00003945563,0.0001549763,0.0002739571,0.003296343],"genre_scores_gemma":[0.9720644,0.0001907059,0.0247291,0.00002335127,0.000007196212,0.00003143281,0.0002143463,0.00005845691,0.002680997],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007492329,"threshold_uncertainty_score":0.002519727,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01334267887999408,"score_gpt":0.242246627904256,"score_spread":0.2289039490242619,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}