{"id":"W3013975168","doi":"10.1007/978-1-0716-0294-2_14","title":"Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania","year":2020,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill Genome Centre; McGill University","funders":"Fonds de Recherche du Québec - Santé; Canadian Institutes of Health Research","keywords":"CRISPR; Genome editing; Biology; Cas9; Guide RNA; Gene; Leishmania; Genome; Computational biology; Trans-activating crRNA; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002325626,0.0003746143,0.0003267986,0.0002987908,0.0003136227,0.0004967786,0.0004156039,0.0004776499,0.001206456],"category_scores_gemma":[0.0001349165,0.0002484974,0.0004639136,0.0002546124,0.0002348029,0.0002108957,0.0005323259,0.0008204315,0.0008189471],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003815064,"about_ca_system_score_gemma":0.0005366228,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001081442,"about_ca_topic_score_gemma":0.001759496,"domain_scores_codex":[0.999706,0.00003868884,0.00003238643,0.00007844012,0.00009978883,0.0000447416],"domain_scores_gemma":[0.9999511,0.000008178631,0.000009670495,0.00001028413,0.00001100004,0.000009762269],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002849705,0.00001910037,0.00009047246,0.0002001212,0.000007209665,0.0001750429,0.0000314853,0.0002800602,0.9891208,0.0006979975,0.0003728322,0.008976391],"study_design_scores_gemma":[0.00001292176,0.0001722543,0.0006804972,0.00002719856,0.00002646901,0.0007837238,0.00003808958,0.001987242,0.9600134,0.0002142887,0.03602269,0.00002122571],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4423537,0.0216417,0.4726966,0.001341455,0.0009326171,0.001457556,0.003655151,0.006106516,0.04981485],"genre_scores_gemma":[0.8005674,0.01119244,0.1689181,0.0004275921,0.00004299959,0.0004399485,0.002252273,0.0002398434,0.01591934],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001206456,"threshold_uncertainty_score":0.004036009,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0131406061236398,"score_gpt":0.3898788883675957,"score_spread":0.3767382822439559,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}