{"id":"W3014711609","doi":"10.1002/ece3.6219","title":"Keeping it cool: Soil sample cold pack storage and DNA shipment up to 1 month does not impact metabarcoding results","year":2020,"lang":"en","type":"article","venue":"Ecology and Evolution","topic":"Environmental DNA in Biodiversity Studies","field":"Environmental Science","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"National Geographic Society; National Science Foundation","keywords":"Amplicon; Sample (material); Environmental science; Amplicon sequencing; Computer science; Cold storage; Soil test; Biochemical engineering; Biology; Ecology; Biotechnology; Soil water; Bacteria; Engineering; Polymerase chain reaction; Gene; Genetics; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005196929,0.0006355797,0.00088246,0.0006954346,0.001900018,0.002383452,0.001082948,0.001160596,0.008572463],"category_scores_gemma":[0.01253702,0.0005015014,0.0006838288,0.001082781,0.001166158,0.001946358,0.00111291,0.001084825,0.002992408],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007887239,"about_ca_system_score_gemma":0.001634772,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004998825,"about_ca_topic_score_gemma":0.01368824,"domain_scores_codex":[0.9956957,0.0009898733,0.0005693535,0.001171669,0.001229279,0.0003440226],"domain_scores_gemma":[0.9902552,0.002690406,0.00208196,0.001865625,0.002705644,0.0004011845],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.003429737,0.0004671722,0.06827977,0.002433669,0.0003809615,0.0005244089,0.001944602,0.0009530627,0.8248103,0.0006228538,0.005921475,0.09023197],"study_design_scores_gemma":[0.00008331317,0.002358997,0.1895907,0.0005777231,0.0005638512,0.001089776,0.001370576,0.001741167,0.7547665,0.001205847,0.04644937,0.0002021058],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9321916,0.004820798,0.04361883,0.002733327,0.001514526,0.0008069191,0.006767613,0.001161561,0.006384866],"genre_scores_gemma":[0.8819348,0.002077411,0.08891179,0.003076833,0.000241923,0.001491661,0.0114987,0.001341157,0.009425681],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008572463,"threshold_uncertainty_score":0.02867776,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02305101834426803,"score_gpt":0.2288266812407217,"score_spread":0.2057756628964537,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}