{"id":"W3015176539","doi":"10.17504/protocols.io.zfef3je","title":"Monitoring cell-surface expression of GPCR by ELISA v1","year":2019,"lang":"en","type":"preprint","venue":"","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"","keywords":"G protein-coupled receptor; Receptor; Cell; Mutant; Cell surface receptor; Cell biology; Surface protein; Molecular biology; Biology; Chemistry; Computational biology; Biochemistry; Virology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005867766,0.001057674,0.000596137,0.0007948049,0.0002309504,0.0006716432,0.0007137351,0.0008103282,0.002376758],"category_scores_gemma":[0.0006526772,0.000289395,0.0003389764,0.0005336758,0.0002090345,0.000406224,0.0004301592,0.001087544,0.004428685],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002422026,"about_ca_system_score_gemma":0.0001523651,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002916471,"about_ca_topic_score_gemma":0.0002847302,"domain_scores_codex":[0.9990677,0.0001976892,0.00005202884,0.0001963188,0.0003249577,0.0001613545],"domain_scores_gemma":[0.9996564,0.0001224186,0.00003957057,0.00005290388,0.00009344357,0.00003526226],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002050651,0.0000213623,0.0001087683,0.00003282849,0.000003996031,0.0000186937,0.000009820074,0.0000712421,0.9979264,0.0001381049,0.0001226628,0.001525657],"study_design_scores_gemma":[0.000003570452,0.00004823347,0.0005499793,0.000004488928,0.000004942127,0.00008664547,0.00001089584,0.001257025,0.9959253,0.0001178183,0.001984917,0.000006149438],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6031721,0.008551727,0.354805,0.0004482605,0.0004347145,0.0004409051,0.007139528,0.003904469,0.02110328],"genre_scores_gemma":[0.7414649,0.01070985,0.20009,0.0005122445,0.0001526322,0.000781513,0.01188199,0.0005336304,0.03387324],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002376758,"threshold_uncertainty_score":0.007951081,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01053566482497927,"score_gpt":0.2444593600627231,"score_spread":0.2339236952377438,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}