{"id":"W3015664414","doi":"10.1007/s00203-020-01873-4","title":"Effect of changes at the conserved + 3 position of mature archaellins on in vitro cleavage by the pre-archaellin peptidase FlaK of Methanococcus maripaludis","year":2020,"lang":"en","type":"article","venue":"Archives of Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Signal peptidase; Signal peptide; Cleavage (geology); Pilin; Biology; Pilus; Biochemistry; Peptide; Amino acid; Conserved sequence; Methanococcus; Peptide sequence; Cell biology; Archaea; Gene; Escherichia coli","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004072364,0.0007383125,0.0005070809,0.0002070171,0.0002589841,0.000706673,0.0006140294,0.0007425121,0.003309062],"category_scores_gemma":[0.0006901868,0.0004908202,0.0003810228,0.0002085086,0.0007180985,0.0004085754,0.0003804447,0.001139068,0.0008081691],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004821162,"about_ca_system_score_gemma":0.0005602076,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001801683,"about_ca_topic_score_gemma":0.001785638,"domain_scores_codex":[0.9995562,0.0001408751,0.00008044551,0.00007089218,0.00006861081,0.00008292762],"domain_scores_gemma":[0.9991191,0.0003189938,0.0002040725,0.00009674211,0.00006533806,0.0001957876],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.003770393,0.0002409642,0.0005968064,0.00007766712,0.00005313806,0.0003547184,0.00006832889,0.0004418952,0.9927395,0.000126019,0.00004246166,0.001488049],"study_design_scores_gemma":[0.0001297343,0.0008250919,0.002752207,0.0000120516,0.00005594008,0.0002549934,0.00007116941,0.0009505679,0.9942924,0.00005260701,0.0005795279,0.00002369963],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9986627,0.0002065672,0.0004973058,0.00005709586,0.00003978502,0.00001603638,0.0001159713,0.00002465151,0.0003798792],"genre_scores_gemma":[0.9986581,0.000102959,0.0005069867,0.00005610956,0.00001063958,0.00000716615,0.0002090437,0.0000296651,0.000419304],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003309062,"threshold_uncertainty_score":0.01106995,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00505928647911438,"score_gpt":0.2162021424195317,"score_spread":0.2111428559404173,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}