{"id":"W3016102097","doi":"10.1101/2020.04.12.038471","title":"Cas9-generated auxotrophs of <i>Phaeodactylum tricornutum</i> are characterized by small and large deletions that can be complemented by plasmid-based genes","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Microbial Community Ecology and Physiology","field":"Environmental Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Phaeodactylum tricornutum; Auxotrophy; Biology; Plasmid; CRISPR; Uracil; Gene; Genetics; Complementation; Biochemistry; Phenotype; DNA; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001218151,0.0005919213,0.0003166005,0.0002410293,0.0002376423,0.000398416,0.0003244652,0.0002656631,0.001691233],"category_scores_gemma":[0.0002095893,0.000192784,0.0002222163,0.000242231,0.0002481168,0.000195167,0.0004595284,0.0004670261,0.0007682483],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003699571,"about_ca_system_score_gemma":0.0002249498,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001054407,"about_ca_topic_score_gemma":0.0016586,"domain_scores_codex":[0.9998046,0.00002498036,0.00002857448,0.00004426567,0.00007432126,0.00002324239],"domain_scores_gemma":[0.9998111,0.00002877709,0.00006772941,0.00003857461,0.00001861362,0.00003524942],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001429682,0.000003659159,0.00007164741,0.00002292021,0.000002711519,0.00003823116,0.000004197139,0.00003618127,0.9991793,0.00005844981,0.00003804305,0.0005302953],"study_design_scores_gemma":[0.000003664228,0.00003507868,0.001580182,0.000005735494,0.000007184843,0.0002812678,0.00001671976,0.0008986294,0.9933271,0.0000305875,0.00380834,0.000005384567],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9519956,0.001039798,0.03528383,0.0002051959,0.0001129951,0.0001346414,0.004358428,0.001131127,0.005738396],"genre_scores_gemma":[0.9723362,0.0006801338,0.01489858,0.00008372745,0.000007848497,0.0001038606,0.005292699,0.0003541519,0.006242708],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001691233,"threshold_uncertainty_score":0.005657673,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0245510427688427,"score_gpt":0.209818758353165,"score_spread":0.1852677155843223,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}