{"id":"W3016385513","doi":"10.1002/aps3.11334","title":"Botanical microbiomes on the cheap: Inexpensive molecular fingerprinting methods to study plant‐associated communities of bacteria and fungi","year":2020,"lang":"en","type":"review","venue":"Applications in Plant Sciences","topic":"Plant Pathogens and Fungal Diseases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":39,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Universidad del Valle; Max-Planck-Gesellschaft","keywords":"Biology; Ribosomal Intergenic Spacer analysis; Terminal restriction fragment length polymorphism; Microbiome; Microbial ecology; Amplicon; Restriction fragment length polymorphism; Temperature gradient gel electrophoresis; Intergenic region; Ribosomal RNA; Genetics; Ribosomal DNA; DNA profiling; Computational biology; Microbial population biology; DNA sequencing; 16S ribosomal RNA; Internal transcribed spacer; Polymerase chain reaction; Bacteria; DNA; Gene; Genome; Phylogenetics","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007292503,0.001018251,0.001383019,0.003340274,0.0002572094,0.001289865,0.0008293224,0.001252299,0.003951898],"category_scores_gemma":[0.0008464959,0.0003268808,0.0005074365,0.002430422,0.0006059573,0.002097321,0.0008576682,0.001866682,0.003625948],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005499204,"about_ca_system_score_gemma":0.0008851456,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009099229,"about_ca_topic_score_gemma":0.001496906,"domain_scores_codex":[0.9997261,0.00004323163,0.00002930493,0.00005675439,0.000121203,0.00002341279],"domain_scores_gemma":[0.9994755,0.0002561933,0.00006955266,0.00002006782,0.0001208482,0.00005786241],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00003997013,0.00004888425,0.0001515724,0.01240622,0.00006278765,0.00009987443,0.0000366653,0.0002080812,0.004061412,0.003374807,0.01663294,0.9628769],"study_design_scores_gemma":[0.000009136572,0.0000732038,0.001018295,0.002709368,0.00007614031,0.0006669635,0.00005774166,0.00007383693,0.0008046608,0.002056106,0.9924275,0.00002712566],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"methods","genre_scores_codex":[0.0001071038,0.9980528,0.0003057876,0.0002931952,0.0002006581,0.000003849008,0.00002212061,0.0000127654,0.001001709],"genre_scores_gemma":[0.0005421804,0.9978691,0.0005358174,0.000240448,0.0001436948,0.000006664603,0.00004067424,0.000003241032,0.0006180857],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003951898,"threshold_uncertainty_score":0.01322043,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06409955767713764,"score_gpt":0.3599164974318079,"score_spread":0.2958169397546702,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}