{"id":"W3016654297","doi":"10.1371/journal.pone.0231664","title":"Highly efficient serum-free manipulation of miRNA in human NK cells without loss of viability or phenotypic alterations is accomplished with TransIT-TKO","year":2020,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Immune Cell Function and Interaction","field":"Immunology and Microbiology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; IWK Health Centre; Canada Foundation for Innovation; Nova Scotia Health Research Foundation","keywords":"microRNA; Transfection; Biology; Cell biology; Phenotype; Cell; Cell culture; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000572932,0.0003546131,0.000503817,0.0001668447,0.0002657918,0.0005533888,0.0003316047,0.0004330073,0.001621966],"category_scores_gemma":[0.0003191896,0.0002181476,0.0003658119,0.0001934718,0.0003992821,0.0002135249,0.000394516,0.0008465996,0.001411907],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002613373,"about_ca_system_score_gemma":0.0003656977,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003478949,"about_ca_topic_score_gemma":0.0007420774,"domain_scores_codex":[0.9994764,0.00009569959,0.00009385521,0.0001501644,0.0001283163,0.00005557339],"domain_scores_gemma":[0.9998513,0.00003863949,0.00003361818,0.0000419751,0.00001630665,0.00001813499],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008541903,0.00002648706,0.00007874972,0.00006160175,0.00000418311,0.00003556675,0.00004308225,0.00006491369,0.9969427,0.0002096285,0.0001382482,0.002309494],"study_design_scores_gemma":[0.00001671608,0.0001554186,0.0002623593,0.000004830023,0.000009957668,0.0001566986,0.000008982647,0.0003830513,0.991163,0.00003359742,0.007801391,0.000004026279],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8586136,0.006378501,0.1172293,0.0006374189,0.0005316467,0.001252725,0.003160699,0.001442612,0.01075359],"genre_scores_gemma":[0.9190576,0.002177922,0.06454461,0.0002174145,0.00004262673,0.0008835843,0.002745046,0.0002739537,0.0100572],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001621966,"threshold_uncertainty_score":0.00542599,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03992545611538003,"score_gpt":0.2321404720121209,"score_spread":0.1922150158967408,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}