{"id":"W3017822470","doi":"10.17504/protocols.io.y4ffytn","title":"Cell lysis, detergent-free v1","year":2019,"lang":"en","type":"preprint","venue":"","topic":"Mass Spectrometry Techniques and Applications","field":"Chemistry","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Lysis; Mass spectrometry; Chromatography; Chemistry; Precipitation; Lysis buffer; Cell; Protein precipitation; Biochemistry; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008165937,0.002312031,0.001863486,0.001373348,0.001059787,0.001970528,0.001920988,0.001283232,0.01798612],"category_scores_gemma":[0.001139717,0.0008352881,0.0007773453,0.00176688,0.0006350786,0.00109209,0.001259965,0.00384135,0.03360952],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005124938,"about_ca_system_score_gemma":0.0008053753,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001039083,"about_ca_topic_score_gemma":0.001236108,"domain_scores_codex":[0.9983159,0.0001823699,0.0001485626,0.0006394015,0.0004439206,0.0002698451],"domain_scores_gemma":[0.9995473,0.00006844466,0.00004376491,0.0001778863,0.00009545425,0.00006720235],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00032437,0.0001276456,0.0001985917,0.0006347722,0.00006143143,0.000175232,0.0001303069,0.0001602607,0.961996,0.004137384,0.01582097,0.0162329],"study_design_scores_gemma":[0.0000549954,0.0001445767,0.0009941643,0.00005912335,0.0000546371,0.0005338215,0.00004004524,0.000925721,0.7580593,0.002245542,0.2368459,0.00004207967],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1428547,0.0213667,0.6351769,0.002282133,0.006030613,0.003479234,0.04473815,0.02613589,0.1179357],"genre_scores_gemma":[0.2094182,0.01915928,0.2705832,0.001882567,0.001070402,0.004108052,0.2196079,0.01096137,0.263209],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01798612,"threshold_uncertainty_score":0.06016958,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01296312942359534,"score_gpt":0.2521307397617892,"score_spread":0.2391676103381938,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}