{"id":"W3020679305","doi":"10.1101/2020.04.26.062786","title":"<i>In vitro</i> Evolution of Antibody Affinity via Insertional Mutagenesis Scanning of an Entire Antibody Variable Region","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"AstraZeneca (Canada)","funders":"Horizon 2020 Framework Programme; Biotechnology and Biological Sciences Research Council; AstraZeneca","keywords":"Indel; Mutagenesis; Point mutation; Genetics; Biology; Computational biology; Affinity maturation; Insertional mutagenesis; Gene; INDEL Mutation; Mutation; Genome","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003955213,0.0003591702,0.000212132,0.0002301093,0.0001530979,0.0004455362,0.000343027,0.0002326074,0.0009640745],"category_scores_gemma":[0.0003377311,0.0001774062,0.0002637568,0.000206355,0.0002973818,0.0001599653,0.0003929016,0.0006897483,0.0005476332],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002688601,"about_ca_system_score_gemma":0.0001786492,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002408832,"about_ca_topic_score_gemma":0.0003746311,"domain_scores_codex":[0.9996153,0.0001134024,0.00003787813,0.00006322858,0.0001134801,0.00005670574],"domain_scores_gemma":[0.9997742,0.00005790156,0.00007614269,0.00004481921,0.00002554043,0.00002149285],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000187841,0.000018833,0.0001095357,0.00001408712,0.000004289343,0.00002020704,0.000007611556,0.0002142061,0.9982526,0.00014664,0.00002352837,0.001169748],"study_design_scores_gemma":[0.000003035606,0.00006575981,0.0002412051,0.000001311621,0.000006719242,0.00009131718,0.000004851518,0.0008388267,0.9979895,0.0000260621,0.0007288965,0.000002433908],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9322679,0.0005302525,0.06288446,0.0001582777,0.00004620269,0.0001115632,0.0003756695,0.0004108516,0.003214816],"genre_scores_gemma":[0.9673128,0.0004282798,0.02980825,0.0001029992,0.00001140461,0.00005004243,0.0004344581,0.0001233821,0.001728457],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009640745,"threshold_uncertainty_score":0.003225148,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02236263284073861,"score_gpt":0.2826580438848877,"score_spread":0.2602954110441491,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}