{"id":"W3021368760","doi":"10.21769/bioprotoc.3599","title":"A Sensitive Coupled Enzyme Assay for Measuring Kinase and ATPase Kinetics Using ADP-Specific Hexokinase","year":2020,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Pancreatic function and diabetes","field":"Medicine","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Biotechnology and Biological Sciences Research Council","keywords":"Hexokinase; Biochemistry; ATPase; Enzyme; Adenosine diphosphate; Adenosine triphosphate; NAD+ kinase; Kinase; Chemistry; Phosphorylation; Glycolysis; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001759126,0.002054245,0.001327755,0.001457313,0.0005252423,0.001574253,0.002172715,0.002309499,0.003264353],"category_scores_gemma":[0.001854569,0.001352124,0.00110023,0.001830565,0.0007586856,0.001510988,0.001502768,0.003645783,0.002562051],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0011484,"about_ca_system_score_gemma":0.0009983578,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007726619,"about_ca_topic_score_gemma":0.001533589,"domain_scores_codex":[0.9958164,0.0007271176,0.0004674453,0.001334077,0.00141685,0.0002380949],"domain_scores_gemma":[0.998693,0.0002978869,0.0002491912,0.0002540587,0.0003238694,0.0001820043],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001407607,0.0001044265,0.0003630754,0.0003737929,0.00006726856,0.00006702977,0.00005641733,0.0002953605,0.9891831,0.0007927875,0.0005293707,0.008026611],"study_design_scores_gemma":[0.00003317652,0.0003340892,0.001334311,0.00005652717,0.00007984306,0.0004785246,0.00003589438,0.003901154,0.9819565,0.000550646,0.01117238,0.00006707296],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07272585,0.007590364,0.9054067,0.0004694509,0.001365787,0.0009907687,0.002701005,0.002661525,0.006088611],"genre_scores_gemma":[0.2468181,0.009064834,0.7122957,0.0007057081,0.0003020355,0.002465473,0.006169042,0.0004532708,0.02172589],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003264353,"threshold_uncertainty_score":0.01092029,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08893067544701089,"score_gpt":0.3116980679454132,"score_spread":0.2227673924984023,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}