{"id":"W3023333636","doi":"10.1021/jacs.6b04032","title":"Single-Molecule Analysis of the Supramolecular Organization of the M<sub>2</sub> Muscarinic Receptor and the Gα<sub>i1</sub> Protein","year":2016,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"University Health Network; University of Toronto","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Heart and Stroke Foundation of Canada; Government of Canada","keywords":"Mastoparan; Chemistry; Random hexamer; Tetramer; G protein; Förster resonance energy transfer; Heterotrimeric G protein; G protein-coupled receptor; Biophysics; Photobleaching; Histone octamer; Green fluorescent protein; Muscarinic acetylcholine receptor; Receptor; Biochemistry; Biology; Fluorescence; Chromatin","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005598767,0.0001760156,0.0004161158,0.00001743343,0.0001316,0.00002147479,0.0007088554,0.000115012,0.00001034177],"category_scores_gemma":[0.0005772249,0.00007071997,0.0009714653,0.000828236,0.0009055352,0.000008204969,0.0003882386,0.000223637,2.706064e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005217292,"about_ca_system_score_gemma":0.0001151916,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001273125,"about_ca_topic_score_gemma":0.000001869778,"domain_scores_codex":[0.9983479,0.0002969585,0.0005156433,0.0002085029,0.0004284379,0.0002025187],"domain_scores_gemma":[0.9976968,0.00007729784,0.00131039,0.0005091073,0.0003467658,0.00005970266],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006242357,0.00005732792,0.0007961133,0.000009095452,0.0006985111,8.278538e-8,0.0001413104,0.00002449172,0.9964656,0.00003075269,0.0003472172,0.001367035],"study_design_scores_gemma":[0.0005755408,0.00008167389,0.0006932337,0.00005805008,0.0005015442,0.000007670999,0.0001692191,0.00004544767,0.9975117,0.00008064189,0.0001699353,0.000105317],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9956031,0.0001757794,0.001983004,0.001926618,0.00008273301,0.0002036781,0.00001351277,0.000002594441,0.00000891149],"genre_scores_gemma":[0.9988707,0.0002313198,0.0002254463,0.0005110911,0.0001185167,0.000003764504,0.000002170175,0.00002483818,0.00001210727],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003267577,"threshold_uncertainty_score":0.3336482,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00417661368959488,"score_gpt":0.1951921095842796,"score_spread":0.1910154958946847,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}