{"id":"W3023333636","doi":"10.1021/jacs.6b04032","title":"Single-Molecule Analysis of the Supramolecular Organization of the M<sub>2</sub> Muscarinic Receptor and the Gα<sub>i1</sub> Protein","year":2016,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"University Health Network; University of Toronto","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Heart and Stroke Foundation of Canada; Government of Canada","keywords":"Mastoparan; Chemistry; Random hexamer; Tetramer; G protein; Förster resonance energy transfer; Heterotrimeric G protein; G protein-coupled receptor; Biophysics; Photobleaching; Histone octamer; Green fluorescent protein; Muscarinic acetylcholine receptor; Receptor; Biochemistry; Biology; Fluorescence; Chromatin","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001385383,0.000208292,0.0002119138,0.0001367213,0.0001668261,0.0002097899,0.0002449322,0.0003487509,0.0008534937],"category_scores_gemma":[0.000175566,0.00009808173,0.000179249,0.0001184706,0.0002051129,0.0002409815,0.0001797287,0.0005105347,0.0002913756],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003246719,"about_ca_system_score_gemma":0.0001147334,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000397013,"about_ca_topic_score_gemma":0.000529894,"domain_scores_codex":[0.9999304,0.000006704918,0.000002899584,0.00002640297,0.00001766993,0.00001596765],"domain_scores_gemma":[0.9999179,0.00002365429,0.00001669173,0.00001386935,0.00001416262,0.00001368867],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001581946,0.000007545939,0.0001371243,0.00001543363,0.000003677703,0.00001632263,0.00000896959,0.00009704626,0.9990729,0.0000704846,0.0000205545,0.0005340536],"study_design_scores_gemma":[0.000004029793,0.00006523237,0.004726812,0.000002597833,0.00001135415,0.0001114017,0.00003286903,0.005681204,0.9885998,0.00008612996,0.0006736817,0.000005035834],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9877335,0.0005136218,0.01042698,0.00007397047,0.0000127967,0.00001773281,0.0002517082,0.00008429489,0.000885292],"genre_scores_gemma":[0.990998,0.0003788646,0.007159164,0.00004353329,0.000006616618,0.00004216881,0.0002813114,0.00001923795,0.001071161],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008534937,"threshold_uncertainty_score":0.002855182,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00417661368959488,"score_gpt":0.1951921095842796,"score_spread":0.1910154958946847,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}