{"id":"W3023544448","doi":"10.21769/bioprotoc.3609","title":"Colorimetric RhoB GTPase Activity Assay","year":2020,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; McGill University Health Centre","funders":"","keywords":"RHOB; GTPase; RHOA; Guanine nucleotide exchange factor; RhoC; RAC1; GTP'; Cell cycle","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001996317,0.004643446,0.00228515,0.003282035,0.001264827,0.001420506,0.002985617,0.001807018,0.01307508],"category_scores_gemma":[0.002093912,0.001823069,0.001600361,0.003367147,0.0008362963,0.001280844,0.001709167,0.006048898,0.01581555],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009701988,"about_ca_system_score_gemma":0.001271004,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009944793,"about_ca_topic_score_gemma":0.002363079,"domain_scores_codex":[0.9910153,0.001585465,0.0006466113,0.001945785,0.004169356,0.0006376526],"domain_scores_gemma":[0.9984393,0.000275701,0.000218637,0.0002206896,0.0006645275,0.0001811853],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002604559,0.0006150233,0.0007115837,0.001296312,0.0001587899,0.0002489626,0.0002872957,0.0007286068,0.9460376,0.003476,0.01435491,0.03182451],"study_design_scores_gemma":[0.00006012696,0.0002570924,0.0009136391,0.0002132296,0.00009366568,0.0005502353,0.00008208121,0.003688197,0.9206557,0.0006827327,0.07269327,0.0001100525],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.0915449,0.04025381,0.6864867,0.001551016,0.002678603,0.003717709,0.01452331,0.02477198,0.1344721],"genre_scores_gemma":[0.2260335,0.03552734,0.5120648,0.001264219,0.0004238365,0.009586016,0.05501513,0.002652616,0.1574326],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01307508,"threshold_uncertainty_score":0.04374051,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03023085610118191,"score_gpt":0.3037378915622569,"score_spread":0.273507035461075,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}