{"id":"W3024076634","doi":"10.1016/j.mex.2020.100918","title":"Application of poxvirus K3 ortholog as a positive selection marker for constructing recombinant vaccinia viruses with modified host range","year":2020,"lang":"en","type":"article","venue":"MethodsX","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Public Health Agency of Canada","funders":"Public Health Agency of Canada","keywords":"Vaccinia; Virus; Biology; Recombinant DNA; Poxviridae; Virology; Orthopoxvirus; Recombinant virus; Viral replication; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005843065,0.000150182,0.0002250209,0.00004538702,0.00008257349,0.00001301947,0.0001408832,0.0001283723,0.00001252745],"category_scores_gemma":[0.0002527306,0.00013334,0.00007969644,0.0002121702,0.0000835871,0.000005517472,0.00004711428,0.0001072633,0.000003325355],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001571431,"about_ca_system_score_gemma":0.0001051652,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005003571,"about_ca_topic_score_gemma":0.00004384448,"domain_scores_codex":[0.9986906,0.0003318046,0.0002206398,0.000412231,0.0001226143,0.0002221186],"domain_scores_gemma":[0.9992296,0.0001016458,0.0001609856,0.0001987175,0.0002293756,0.00007974279],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.01257316,0.00003954246,0.006085104,0.00003693405,0.000117018,9.729883e-7,0.0001269338,0.00003922308,0.954514,0.00007473344,0.00002944065,0.02636296],"study_design_scores_gemma":[0.00145247,0.004786862,0.002672345,0.000009174782,0.00003926901,0.00001801044,0.0001100604,0.000648822,0.989302,0.000113624,0.0006763238,0.0001710112],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8490048,0.0001644173,0.1495481,0.0002327473,0.000028774,0.000735722,0.00003171943,0.00001761639,0.0002360426],"genre_scores_gemma":[0.9402114,0.00003832753,0.05887036,0.0004871152,0.00009738463,0.0001811852,0.00003558228,0.00003893055,0.00003977973],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0912065,"threshold_uncertainty_score":0.5437446,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03677701555221681,"score_gpt":0.35386827762113,"score_spread":0.3170912620689132,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}