{"id":"W3024649835","doi":"10.3389/fmicb.2020.00881","title":"Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression","year":2020,"lang":"en","type":"article","venue":"Frontiers in Microbiology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"H2020 Marie Skłodowska-Curie Actions; Deutsche Forschungsgemeinschaft; Technische Universität Berlin; Shiraz University; European Commission","keywords":"PMSF; Psychrophile; Biochemistry; Protease; Proteases; Bacteria; Biology; Enzyme; Thermus; Microbiology; Thermophile; Chemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00004168654,0.0001263071,0.0003061794,0.0001176218,0.00001868845,0.00001253589,0.00006693848,0.0001319736,0.000007407045],"category_scores_gemma":[0.00001129001,0.0001278477,0.00002730874,0.0002003049,0.00008456886,0.00003094667,0.00006442134,0.00007569628,2.200349e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001297401,"about_ca_system_score_gemma":0.00002670419,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003105289,"about_ca_topic_score_gemma":0.00001372223,"domain_scores_codex":[0.9990664,0.0001482305,0.0002279729,0.0004242703,0.00002041129,0.0001126924],"domain_scores_gemma":[0.9996479,0.000002904087,0.000144338,0.0001215753,0.00002442173,0.00005891683],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001116802,0.00004793073,0.02016554,0.00001190326,0.00006065024,8.392191e-7,0.0009586735,0.000006142353,0.9772927,2.222566e-7,0.00001294194,0.0003256321],"study_design_scores_gemma":[0.0005365094,0.00009543113,0.1264801,0.000004924178,0.00002881795,0.000001197038,0.0001381179,0.000188615,0.8718525,0.000006049655,0.0005520681,0.000115712],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9735614,0.0001928779,0.0254614,0.0001933228,0.00006687026,0.0002632919,0.0002551251,0.000004132889,0.000001598449],"genre_scores_gemma":[0.9940488,0.0001291605,0.001801344,0.0001964034,0.0000852962,0.00001688066,0.003688404,0.000008195298,0.00002553137],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1063146,"threshold_uncertainty_score":0.5213479,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009615070489407409,"score_gpt":0.223081744989068,"score_spread":0.2134666744996606,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}