{"id":"W3027306138","doi":"10.3791/61053","title":"A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes","year":2020,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Mass Spectrometry Techniques and Applications","field":"Chemistry","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Hydrogen–deuterium exchange; Chemistry; Peptide; Enzyme; Deuterium; Mass spectrometry; Allosteric regulation; Protein dynamics; Protein structure; Substrate (aquarium); Biophysics; Biochemistry; Chromatography","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006508348,0.0006797453,0.0004874413,0.0005784801,0.0005420969,0.0009216237,0.0006921451,0.000977769,0.001680094],"category_scores_gemma":[0.0002531875,0.0003305732,0.000339345,0.0003789151,0.0004652499,0.0009846827,0.000728945,0.001708109,0.00105498],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004245152,"about_ca_system_score_gemma":0.0004044485,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000408932,"about_ca_topic_score_gemma":0.0006995162,"domain_scores_codex":[0.9997194,0.00003526038,0.00001364055,0.00009830221,0.0001001522,0.00003332504],"domain_scores_gemma":[0.9998358,0.00003833834,0.0000291897,0.00002364053,0.00002436146,0.00004865891],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004868595,0.00002463557,0.0002307384,0.00006668404,0.000008486556,0.00007098771,0.00001555539,0.0001527054,0.993257,0.0005654797,0.0004677439,0.005091344],"study_design_scores_gemma":[0.00002708323,0.0002021798,0.001823892,0.00001331893,0.00001742366,0.00052257,0.00002491857,0.005141142,0.9697992,0.0007145096,0.02168853,0.00002523515],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.543502,0.02046948,0.4041703,0.002941585,0.001141702,0.0007030556,0.008269398,0.004941487,0.01386099],"genre_scores_gemma":[0.4916447,0.01218279,0.4719264,0.001136246,0.0002812578,0.0004331727,0.006027408,0.0004696864,0.01589836],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001680094,"threshold_uncertainty_score":0.005620539,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0513772577566769,"score_gpt":0.3810476063420575,"score_spread":0.3296703485853806,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}