{"id":"W3027490964","doi":"10.1007/s00253-020-10669-x","title":"Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris","year":2020,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Akademie Věd České Republiky; SCIEX","keywords":"Pichia pastoris; Intracellular; Extracellular; Heterologous; Secretion; Secretory protein; Biology; Secretory pathway; Biochemistry; Cell biology; Recombinant DNA; Cell","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000241473,0.0003780372,0.0004904764,0.0003345053,0.0001436621,0.0006373737,0.0001896856,0.000305176,0.0002694869],"category_scores_gemma":[0.0003947254,0.0002107582,0.0003133146,0.0004742506,0.0001604222,0.0003133505,0.0001873692,0.0004509397,0.0002269559],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004927936,"about_ca_system_score_gemma":0.000176207,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002812431,"about_ca_topic_score_gemma":0.0009705959,"domain_scores_codex":[0.9997641,0.00003891902,0.00004032448,0.00006105126,0.00006996851,0.00002565457],"domain_scores_gemma":[0.9998023,0.00005776023,0.00004089389,0.00003471126,0.00003545125,0.00002900553],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001240513,0.00002962594,0.001011271,0.00006583718,0.00001601691,0.00008138145,0.0000462036,0.001045786,0.9948849,0.00007265465,0.0000560155,0.002566251],"study_design_scores_gemma":[0.00002263674,0.0004617673,0.01646967,0.00001438034,0.00004252168,0.0001727324,0.00005909341,0.02896661,0.951702,0.00007753317,0.001980604,0.00003027819],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9940732,0.001140089,0.003247559,0.00005379966,0.00002230175,0.0000202669,0.0006533976,0.0001173596,0.000671997],"genre_scores_gemma":[0.9940993,0.0006841396,0.00337587,0.00002214128,0.00000642632,0.00002564947,0.0008578407,0.00002631808,0.0009024049],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002812431,"threshold_uncertainty_score":0.005592167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004992912942879344,"score_gpt":0.1996763358823696,"score_spread":0.1946834229394902,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}