{"id":"W3034739500","doi":"10.1186/s13059-020-02051-x","title":"SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds","year":2020,"lang":"en","type":"article","venue":"Genome biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":77,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Genetics","funders":"Chinese Academy of Sciences; Institute of Microbiology, Chinese Academy of Sciences; National Natural Science Foundation of China","keywords":"Genome editing; CRISPR; Biology; Cas9; Cytidine deaminase; RNA editing; Computational biology; Guide RNA; Indel; Genetics; RNA; DNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000106487,0.0002334018,0.0002581135,0.00006771206,0.00004264801,0.00001359974,0.000152861,0.0001960953,0.0000244495],"category_scores_gemma":[0.00004298769,0.0002152771,0.00004079213,0.00009346569,0.00006945632,0.000003295486,0.0001349118,0.0001043873,0.000006671867],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001180654,"about_ca_system_score_gemma":0.00004288195,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003008607,"about_ca_topic_score_gemma":0.0001235642,"domain_scores_codex":[0.9987383,0.00003674236,0.0002458729,0.0005057614,0.00005764866,0.0004156732],"domain_scores_gemma":[0.9995323,0.00001713286,0.00005110316,0.0001846227,0.00002763551,0.0001871781],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001280411,0.00001770218,0.006451679,0.00003358856,0.00004945746,0.00003261647,0.0002422478,0.001264549,0.9911644,0.00001620163,0.00002718958,0.0005723165],"study_design_scores_gemma":[0.01504987,0.006286745,0.344064,0.0001039514,0.0001460335,0.000819082,0.002294042,0.006783446,0.498025,0.0001057221,0.1225783,0.003743882],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9912026,0.00366539,0.004280199,0.0002867957,0.00008287669,0.0002130222,0.0001411088,0.00002536475,0.0001026537],"genre_scores_gemma":[0.996885,0.0002367679,0.001497822,0.0003268512,0.0005542828,0.00002635701,0.0004190649,0.00003280554,0.00002101757],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4931394,"threshold_uncertainty_score":0.8778747,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009938021214321496,"score_gpt":0.2662095425051744,"score_spread":0.256271521290853,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}