{"id":"W3036865653","doi":"10.1021/acssensors.0c00279","title":"Bright and High-Performance Genetically Encoded Ca <sup>2+</sup> Indicator Based on mNeonGreen Fluorescent Protein","year":2020,"lang":"en","type":"article","venue":"ACS Sensors","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Japan Society for the Promotion of Science; Canadian Institutes of Health Research","keywords":"Green fluorescent protein; Zebrafish; Aequorea victoria; Biophysics; Fluorescence; Chemistry; In vivo; Fluorescent protein; Cell biology; Biology; Biochemistry; Physics; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003005909,0.0004133831,0.0003269594,0.0001993707,0.0002073984,0.0004779732,0.0006629818,0.0006010993,0.0007224221],"category_scores_gemma":[0.0003341479,0.0001699601,0.0001887377,0.0002839064,0.0004713092,0.0006357584,0.0004783524,0.0008442344,0.0005798598],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009292419,"about_ca_system_score_gemma":0.0004734987,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00196706,"about_ca_topic_score_gemma":0.003851873,"domain_scores_codex":[0.9998083,0.00001353596,0.00000807961,0.00005492804,0.00008235317,0.00003288049],"domain_scores_gemma":[0.9998123,0.00003125205,0.00004030163,0.00001688986,0.00005013873,0.0000491504],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000201008,0.000003940017,0.00009059868,0.00002587727,0.000001724131,0.0000291105,0.000008415738,0.00007152327,0.9977869,0.0003045091,0.0001257337,0.001531545],"study_design_scores_gemma":[0.000002868504,0.00002601328,0.0004699294,0.000004333319,0.00000386688,0.00009759043,0.000009273111,0.0008645287,0.9943936,0.00005519126,0.004066447,0.000006347693],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.829895,0.004617519,0.1479963,0.001352495,0.0002015432,0.0002116611,0.001744347,0.001921977,0.01205914],"genre_scores_gemma":[0.7888767,0.003417476,0.1939114,0.0003026767,0.00002444891,0.0001489081,0.001722643,0.0003634079,0.01123241],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00196706,"threshold_uncertainty_score":0.00674212,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006382321903291454,"score_gpt":0.2237799277644592,"score_spread":0.2173976058611677,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}