{"id":"W3037538172","doi":"10.1093/nar/gkaa516","title":"A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase","year":2020,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"DNA and Nucleic Acid Chemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":703,"is_retracted":false,"has_abstract":true,"ca_institutions":"Wilfrid Laurier University","funders":"Folkhälsan Research Center, University of Helsinki; Lastentautien Tutkimussäätiö; Magnus Ehrnroothin Säätiö; Biomedicum Helsinki-säätiö; Medicinska Understödsföreningen Liv och Hälsa","keywords":"Biology; DNA polymerase; Oligonucleotide; DNA; DNA synthesis; DNA replication; Molecular biology; Taq polymerase; Polymerase; Biochemistry; Deoxyribonucleoside; Primer (cosmetics); Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003397397,0.003760534,0.001778924,0.003228084,0.0008400872,0.001541586,0.002769987,0.002633045,0.003558466],"category_scores_gemma":[0.003699123,0.001703331,0.001440096,0.001430182,0.001675797,0.001584673,0.001857049,0.00392874,0.005147099],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001026473,"about_ca_system_score_gemma":0.001319452,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004221862,"about_ca_topic_score_gemma":0.001227897,"domain_scores_codex":[0.9916965,0.002375591,0.0007513119,0.002837992,0.002026341,0.000312206],"domain_scores_gemma":[0.9960904,0.001861883,0.000641809,0.0006227915,0.0005154145,0.000267667],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005540402,0.00006707577,0.0003618839,0.0007146425,0.00004695267,0.00006873017,0.00009874027,0.0002062203,0.9867271,0.0008268834,0.0003252665,0.01050121],"study_design_scores_gemma":[0.000009264924,0.0001596837,0.0005885225,0.00006013306,0.00004512103,0.0004762303,0.00002859464,0.001775532,0.9882352,0.0004132864,0.008176318,0.0000320891],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.02621905,0.006693894,0.9601445,0.0002124736,0.0004763864,0.0005082259,0.0009954942,0.002287525,0.002462403],"genre_scores_gemma":[0.06123735,0.004354513,0.919017,0.0002731813,0.0001186598,0.001446506,0.002685534,0.0003015939,0.01056565],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003760534,"threshold_uncertainty_score":0.01796734,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02473192658312632,"score_gpt":0.2966230524759356,"score_spread":0.2718911258928092,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}