{"id":"W3038425740","doi":"","title":"Optimization of Chitosan DNA Nanoparticles for Gene Delivery: DNA concentration, ionic strength and N:P ratio are key determinants of transfection efficiency.","year":2020,"lang":"en","type":"article","venue":"Pharmaceutics","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University","funders":"","keywords":"Transfection; Chitosan; Gene delivery; DNA; Ionic strength; Chemistry; Nanoparticle; Gene; Nanotechnology; Biochemistry; Materials science; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006900583,0.0006866627,0.000353856,0.0003154685,0.0002682546,0.0005361185,0.0004340331,0.0005076922,0.0006048491],"category_scores_gemma":[0.0009353653,0.0002481198,0.0002663202,0.000364769,0.0003711405,0.0004246508,0.0002310984,0.0003635319,0.0002475803],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007460155,"about_ca_system_score_gemma":0.0007588034,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002298213,"about_ca_topic_score_gemma":0.005570414,"domain_scores_codex":[0.9994648,0.0001061435,0.00008053934,0.0001164003,0.0001741588,0.00005798221],"domain_scores_gemma":[0.9996069,0.0001336837,0.0001027796,0.00001792244,0.00009959898,0.00003900832],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007008085,0.00002005414,0.00008415028,0.00005449263,0.00000594746,0.00002376392,0.00001225039,0.000173421,0.9983542,0.00004496888,0.00003104896,0.001125617],"study_design_scores_gemma":[0.000008456644,0.000076551,0.0004403992,0.0000046281,0.00001126784,0.00003051931,0.00000645695,0.0006729179,0.998105,0.00001611515,0.0006199041,0.00000765133],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9694802,0.002668696,0.02348267,0.0002734712,0.00008140501,0.0004240961,0.0003236047,0.0001969326,0.00306894],"genre_scores_gemma":[0.9645663,0.002199947,0.0301524,0.0001028951,0.00001636328,0.0002875354,0.0002764563,0.00008488126,0.002313172],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002298213,"threshold_uncertainty_score":0.005412698,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02895268152942382,"score_gpt":0.2880577961151392,"score_spread":0.2591051145857154,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}