{"id":"W3038425740","doi":"","title":"Optimization of Chitosan DNA Nanoparticles for Gene Delivery: DNA concentration, ionic strength and N:P ratio are key determinants of transfection efficiency.","year":2020,"lang":"en","type":"article","venue":"Pharmaceutics","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University","funders":"","keywords":"Transfection; Chitosan; Gene delivery; DNA; Ionic strength; Chemistry; Nanoparticle; Gene; Nanotechnology; Biochemistry; Materials science; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00004630427,0.00009459141,0.000136329,0.00001442024,0.0000474939,0.000009298993,0.00007188797,0.0000591061,0.000004697501],"category_scores_gemma":[0.00003677256,0.00009559294,0.00004705107,0.00006659546,0.00006377985,0.000008970403,0.00001785167,0.00002894278,1.835214e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000002650466,"about_ca_system_score_gemma":0.00004686031,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003046971,"about_ca_topic_score_gemma":0.000006445691,"domain_scores_codex":[0.999348,0.00003292185,0.0002487271,0.0001813494,0.00007340643,0.0001155812],"domain_scores_gemma":[0.9996403,0.00001285608,0.0001267627,0.00007636922,0.0000842717,0.00005946017],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001919546,0.00006020467,0.00328976,0.00007898192,0.00002973733,2.957779e-7,0.0002464407,0.01031935,0.9842253,0.000005028292,0.00001727488,0.001535616],"study_design_scores_gemma":[0.0007639964,0.0003858657,0.0004278026,0.00001357285,0.00006547068,0.000002101425,0.000100326,0.09006592,0.908023,0.00000194392,0.00006266173,0.00008732128],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9876727,0.000629573,0.01119614,0.00007974826,0.00007089123,0.0002468003,0.00008667558,0.000006006306,0.00001142765],"genre_scores_gemma":[0.9980749,0.001108921,0.0005418807,0.0001235644,0.00006786573,0.000009938273,0.0000613863,0.00000945001,0.000002091368],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07974657,"threshold_uncertainty_score":0.3898167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02895268152942382,"score_gpt":0.2880577961151392,"score_spread":0.2591051145857154,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}