{"id":"W3038946788","doi":"10.3791/61401","title":"Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time","year":2021,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Trinity College","funders":"Medical Research Council; Biotechnology and Biological Sciences Research Council; Directorate for Biological Sciences; Wellcome Trust","keywords":"Nucleotide; Biophysics; Membrane protein; Gating; Ion channel; Membrane; Biochemistry; Ligand (biochemistry); Förster resonance energy transfer; Chemistry; G protein-coupled receptor; Biology; Fluorescence; Cell biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003217326,0.0001386447,0.0002644054,0.0001666033,0.00004584388,0.00003159175,0.0001075492,0.00009101135,0.00002180751],"category_scores_gemma":[0.0001474791,0.0001249564,0.0001481927,0.0002271641,0.00002093072,0.00001176924,0.00009231685,0.0001104607,0.000008839424],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008067723,"about_ca_system_score_gemma":0.0001014558,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007445841,"about_ca_topic_score_gemma":0.000005567901,"domain_scores_codex":[0.9987472,0.0001081916,0.0004464174,0.0002145784,0.0002905527,0.0001931188],"domain_scores_gemma":[0.999307,0.000008868397,0.0002078218,0.0001598325,0.0002126041,0.0001038931],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003116209,0.0001812398,0.0003932062,0.000005043612,0.0000891847,0.00006070732,0.00005372457,0.00001747312,0.9981613,0.000006942438,0.0003131815,0.0004064329],"study_design_scores_gemma":[0.000762696,0.0002218045,0.0003382805,0.00009907502,0.00001577458,0.00007893259,0.0001302714,0.00001469607,0.995717,0.00001171725,0.002466948,0.0001427933],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980557,0.0001200383,0.0009738366,0.00008417297,0.00009325532,0.0000881965,0.000002923655,0.00001035398,0.0005715043],"genre_scores_gemma":[0.978472,0.0001091845,0.02044838,0.0001823011,0.0002247035,0.000003588287,0.00001860714,0.00002197356,0.000519294],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01958375,"threshold_uncertainty_score":0.5095573,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0342303841813771,"score_gpt":0.3758259454379133,"score_spread":0.3415955612565362,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}