{"id":"W3040319237","doi":"10.3791/61421","title":"Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics","year":2020,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Multiplex; Epigenetics; DNA methylation; CpG site; Methylation; Biology; Bisulfite; Molecular biology; Computational biology; Peripheral blood mononuclear cell; DNA; Genetics; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00102932,0.0008819191,0.0005849414,0.0006297267,0.0002920233,0.0005473836,0.0007760659,0.0008678958,0.003495462],"category_scores_gemma":[0.000849675,0.0006595976,0.0004350071,0.0003954907,0.0003867532,0.0004729308,0.0006420041,0.001042839,0.001621811],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003576869,"about_ca_system_score_gemma":0.0004260702,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001334396,"about_ca_topic_score_gemma":0.0002551285,"domain_scores_codex":[0.9987465,0.0001959072,0.00007857542,0.0005411306,0.0003622921,0.00007554514],"domain_scores_gemma":[0.9997087,0.0001212311,0.00004764903,0.00004335689,0.00005634134,0.00002260625],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004834969,0.00003251972,0.0001695247,0.0001177251,0.000008866562,0.00005473009,0.00003383552,0.000202902,0.9864129,0.0005304792,0.0003082588,0.01207996],"study_design_scores_gemma":[0.00001629578,0.0001747215,0.0004965222,0.00001154029,0.00001449526,0.0002409253,0.00001005552,0.005659826,0.9837388,0.0003028528,0.009308513,0.00002543986],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.073778,0.002153616,0.9129641,0.0003023798,0.0004333647,0.00153525,0.00172105,0.003425228,0.003686999],"genre_scores_gemma":[0.1488912,0.001627142,0.8371285,0.0003413739,0.00008604156,0.002621838,0.001590605,0.0002068479,0.007506514],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003495462,"threshold_uncertainty_score":0.01169348,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09747777815326017,"score_gpt":0.3974960991363305,"score_spread":0.3000183209830704,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}