{"id":"W3040319237","doi":"10.3791/61421","title":"Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics","year":2020,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Multiplex; Epigenetics; DNA methylation; CpG site; Methylation; Biology; Bisulfite; Molecular biology; Computational biology; Peripheral blood mononuclear cell; DNA; Genetics; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001615742,0.000213567,0.0003805005,0.00004689608,0.0001051262,0.00005237592,0.0002058352,0.0001831792,0.00004428045],"category_scores_gemma":[0.0001840502,0.0001865422,0.0002522374,0.00009513776,0.00004905546,0.00001343277,0.00004592542,0.00011119,0.000002914159],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002994876,"about_ca_system_score_gemma":0.000074319,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005264024,"about_ca_topic_score_gemma":6.584631e-7,"domain_scores_codex":[0.9985443,0.0001090712,0.0006197728,0.0002523778,0.0002283848,0.0002461308],"domain_scores_gemma":[0.9990516,0.00004990387,0.0003639527,0.0001276942,0.000191024,0.0002158287],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007377663,0.0002269197,0.001293403,0.00002126045,0.000116356,0.00001280345,0.0002685691,0.00006620873,0.995433,0.00003910382,0.001412872,0.0003717583],"study_design_scores_gemma":[0.003031764,0.0006563452,0.0000515953,0.00002216571,0.000051065,0.0000299048,0.0001651495,0.002344793,0.9752004,0.000009255139,0.01821857,0.0002190506],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8960828,0.005751452,0.09731766,0.0001365379,0.0004430208,0.0002186944,0.00002730239,0.000007887453,0.00001466365],"genre_scores_gemma":[0.98167,0.0004435497,0.01575677,0.0009972194,0.001012892,0.000007727255,0.00005302389,0.00004372091,0.0000150676],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08558725,"threshold_uncertainty_score":0.7606969,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09747777815326017,"score_gpt":0.3974960991363305,"score_spread":0.3000183209830704,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}