{"id":"W3042652718","doi":"10.1016/j.bbrc.2020.06.056","title":"Ligand specificity and affinity in the sulforhodamine B binding RNA aptamer","year":2020,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Sulforhodamine B; Tetramer; Chemistry; Binding selectivity; Ligand (biochemistry); Selectivity; Binding site; Combinatorial chemistry; Biophysics; Stereochemistry; Biochemistry; Biology; Molecular biology; Receptor; Cytotoxicity","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004481724,0.0001832117,0.0002480316,0.0002302311,0.0002516067,0.0004674987,0.0003198232,0.0003972014,0.001546455],"category_scores_gemma":[0.0005534819,0.0002045645,0.0001280198,0.0001495121,0.0002496823,0.0001991507,0.0001407641,0.0004215289,0.0005203032],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003529436,"about_ca_system_score_gemma":0.0002526859,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001825597,"about_ca_topic_score_gemma":0.002489095,"domain_scores_codex":[0.9995328,0.0001063026,0.0000251083,0.0001142685,0.000138633,0.0000828889],"domain_scores_gemma":[0.9997378,0.000130927,0.00002520237,0.00001816769,0.0000413571,0.00004654957],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005942911,0.000007802581,0.0002083699,0.00001124185,0.000001799721,0.00001590027,0.00001278378,0.00009620857,0.9988914,0.0001202389,0.00002470873,0.0005500983],"study_design_scores_gemma":[0.00000742633,0.00006707336,0.002005187,0.000002012112,0.000006195459,0.0001405541,0.0000152395,0.001933565,0.9953242,0.00004484638,0.0004475096,0.000006142639],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.993701,0.0004759463,0.003533359,0.00009072694,0.000008217098,0.000007520928,0.00009205703,0.0000743253,0.002016827],"genre_scores_gemma":[0.9972087,0.00009565845,0.001289387,0.00006136022,0.000002877829,0.000005165273,0.0001143323,0.00001724119,0.001205466],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001825597,"threshold_uncertainty_score":0.005173385,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1035703771293388,"score_gpt":0.3824116982568697,"score_spread":0.2788413211275309,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}