{"id":"W3044261814","doi":"10.3390/ijms21186466","title":"A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?","year":2020,"lang":"en","type":"review","venue":"International Journal of Molecular Sciences","topic":"Extracellular vesicles in disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":799,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Children's Hospital Research Institute of Manitoba; Research Manitoba","funders":"Children's Hospital Research Institute of Manitoba; University of Manitoba; Research Manitoba","keywords":"Microvesicles; Exosome; Extracellular vesicles; Vesicle; Ultrafiltration (renal); Nanoparticle tracking analysis; Size-exclusion chromatography; Computational biology; Ultracentrifuge; Cell biology; Biology; Lysis; Isolation (microbiology); Ethylene glycol; Chemistry; Biochemistry; Membrane; Bioinformatics; microRNA; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001387872,0.001230868,0.001997985,0.003152244,0.0003455158,0.00150267,0.001187635,0.00134542,0.002547146],"category_scores_gemma":[0.001859856,0.0004740364,0.0008586351,0.002949298,0.0006048869,0.002539067,0.0007091052,0.001462964,0.003022734],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005850613,"about_ca_system_score_gemma":0.0011899,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006634804,"about_ca_topic_score_gemma":0.000689469,"domain_scores_codex":[0.9992958,0.0001251964,0.0001597427,0.0001133137,0.0002625795,0.00004337314],"domain_scores_gemma":[0.9988128,0.0005953751,0.0001570655,0.00004151926,0.0003311812,0.00006211518],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001341349,0.00009649516,0.000316949,0.0505248,0.0001944939,0.0003353512,0.0001030754,0.0003866383,0.01063285,0.003476933,0.03581797,0.8979804],"study_design_scores_gemma":[0.00001388292,0.0001869308,0.000846842,0.005484432,0.0002161438,0.001418513,0.00007985281,0.0001855745,0.005010934,0.001363451,0.9851306,0.0000628174],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.0002091204,0.9975623,0.0008604285,0.0003745458,0.0003713688,0.00001231061,0.00005112873,0.00002447832,0.000534271],"genre_scores_gemma":[0.0006399312,0.9971179,0.001064921,0.0004022247,0.0002577605,0.00002032616,0.00009391634,0.000008043688,0.0003950042],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.003152244,"threshold_uncertainty_score":0.00852102,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0317829292253417,"score_gpt":0.3988691723063032,"score_spread":0.3670862430809615,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}