{"id":"W3044420264","doi":"10.1128/aem.01040-20","title":"Engineering Burkholderia xenovorans LB400 BphA through Site-Directed Mutagenesis at Position 283","year":2020,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institut National de la Recherche Scientifique","funders":"Chinese Academy of Sciences Key Project; Chinese Academy of Sciences; National Natural Science Foundation of China","keywords":"Mutagenesis; Biology; Site-directed mutagenesis; Chemistry; Microbiology; Mutation; Genetics; Mutant; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002007521,0.0003402603,0.000190075,0.0001635252,0.0001323065,0.0002714826,0.0003356767,0.0002490651,0.0005830826],"category_scores_gemma":[0.0002559178,0.0001446582,0.0002002883,0.0002645668,0.0002116758,0.0001534441,0.000313059,0.0005225883,0.0002613811],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002772908,"about_ca_system_score_gemma":0.00032945,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001594871,"about_ca_topic_score_gemma":0.001293385,"domain_scores_codex":[0.9998167,0.00004360142,0.00002041695,0.00002789001,0.00005425969,0.00003717538],"domain_scores_gemma":[0.9998752,0.00003041895,0.00004169586,0.00001798369,0.00001331501,0.00002127395],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001234266,0.00007765793,0.0003575632,0.00003777288,0.00000896951,0.0001232031,0.00003630643,0.0004503246,0.9961307,0.0002435629,0.00002623367,0.002384201],"study_design_scores_gemma":[0.00002299641,0.0002530891,0.001971779,0.000006550091,0.00001853918,0.0002109993,0.00007466402,0.003385978,0.9911982,0.0001035643,0.002743614,0.000009872691],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9907612,0.0001624894,0.007884849,0.00006456467,0.00001903391,0.00005473835,0.0001925842,0.00006969804,0.0007909091],"genre_scores_gemma":[0.9916883,0.0001731517,0.0065116,0.00002288711,0.000003997498,0.00003185869,0.0002844386,0.00004550565,0.001238181],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001594871,"threshold_uncertainty_score":0.003171146,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005300165376917112,"score_gpt":0.1647760345755812,"score_spread":0.1594758691986641,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}