{"id":"W3044710522","doi":"10.1016/j.molcel.2020.07.005","title":"Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity","year":2020,"lang":"en","type":"article","venue":"Molecular Cell","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":166,"is_retracted":false,"has_abstract":false,"ca_institutions":"Institute of Genetics","funders":"National Transgenic Science and Technology Program; Youth Innovation Promotion Association of the Chinese Academy of Sciences; National Natural Science Foundation of China; United Soybean Board","keywords":"Biology; Cas9; Cytidine deaminase; Computational biology; Genome; genomic DNA; Cytosine; Genetics; Cytidine; DNA; Gene; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006022353,0.0007296751,0.0005926512,0.0002659991,0.0003493798,0.001127215,0.0007911228,0.001152849,0.003346539],"category_scores_gemma":[0.0009785388,0.0004480621,0.0003298947,0.0002516653,0.000379121,0.0004506544,0.0004810182,0.001643267,0.001642366],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009389525,"about_ca_system_score_gemma":0.0007152447,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009567979,"about_ca_topic_score_gemma":0.003014349,"domain_scores_codex":[0.9994312,0.0001160489,0.00005420293,0.000147803,0.0001501437,0.0001006013],"domain_scores_gemma":[0.9996531,0.00009724422,0.0000739118,0.00004476672,0.00007185495,0.00005912437],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002038998,0.0001013303,0.0001355816,0.0001309871,0.00002910072,0.0002584144,0.00003569492,0.00132368,0.9853017,0.002431358,0.0007193271,0.009329026],"study_design_scores_gemma":[0.0000423208,0.00008963732,0.00006763367,0.000006789984,0.0000183278,0.0001745849,0.00001122385,0.002544784,0.9894887,0.0001865831,0.007351378,0.00001815585],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7386284,0.004514697,0.217601,0.001444833,0.001652869,0.0008529435,0.001467046,0.004632792,0.02920532],"genre_scores_gemma":[0.8896883,0.0008624161,0.0946197,0.000521293,0.00005705255,0.0001697419,0.0005035655,0.0003835386,0.01319439],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003346539,"threshold_uncertainty_score":0.01119524,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005181607873979125,"score_gpt":0.2164771484335223,"score_spread":0.2112955405595432,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}