{"id":"W3046653028","doi":"10.1016/j.scr.2020.101930","title":"Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system","year":2020,"lang":"en","type":"article","venue":"Stem Cell Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research; Natural Science Foundation of Beijing Municipality; National Natural Science Foundation of China","keywords":"Biology; Embryonic stem cell; Cell biology; CRISPR; Stem cell; Germline; Germ layer; Myofilament; Molecular biology; Genetics; Gene; Myocyte; Induced pluripotent stem cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0006948475,0.0002955706,0.0003582122,0.0001212494,0.0002143566,0.00007224762,0.0005194345,0.0002959485,0.00002469518],"category_scores_gemma":[0.000008959651,0.0002996423,0.0001588781,0.0003302802,0.00009798326,0.00000778857,0.0001877063,0.0003696116,0.00003397817],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007061174,"about_ca_system_score_gemma":0.0002440768,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006244517,"about_ca_topic_score_gemma":0.00004155303,"domain_scores_codex":[0.9970814,0.0003753226,0.0004864538,0.0007759646,0.000640141,0.0006407239],"domain_scores_gemma":[0.9983632,0.00004000121,0.0001069389,0.0007378306,0.0003675807,0.0003844392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001100496,0.0001446466,0.0000867671,0.0007785145,0.00002316269,0.00002268808,0.0001587518,0.003663796,0.9912297,0.0000186678,0.002510944,0.001252292],"study_design_scores_gemma":[0.001062496,0.002030074,0.00005791242,0.00003212172,0.00002282605,0.000005191608,0.0005838615,0.01704955,0.9735273,7.057432e-7,0.005335995,0.0002919653],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9832902,0.004286511,0.01030901,0.0001376285,0.0001578604,0.000643443,0.0001083034,0.00005163917,0.001015402],"genre_scores_gemma":[0.9968243,0.00006099481,0.0001593974,0.00003909537,0.0006358906,0.00006050468,0.0003345597,0.00008238121,0.001802938],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01770242,"threshold_uncertainty_score":0.9999456,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06562680782180927,"score_gpt":0.3576540535833208,"score_spread":0.2920272457615115,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}