{"id":"W3046653028","doi":"10.1016/j.scr.2020.101930","title":"Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system","year":2020,"lang":"en","type":"article","venue":"Stem Cell Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research; Natural Science Foundation of Beijing Municipality; National Natural Science Foundation of China","keywords":"Biology; Embryonic stem cell; Cell biology; CRISPR; Stem cell; Germline; Germ layer; Myofilament; Molecular biology; Genetics; Gene; Myocyte; Induced pluripotent stem cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002467772,0.0003035245,0.0003567432,0.0003681926,0.0003408054,0.0002775381,0.0004217353,0.0004320199,0.002240424],"category_scores_gemma":[0.0001117333,0.0002459091,0.0002844572,0.0002704699,0.0002317631,0.0001629776,0.0003208166,0.0007356514,0.001164203],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001803508,"about_ca_system_score_gemma":0.0002507618,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009037249,"about_ca_topic_score_gemma":0.001230885,"domain_scores_codex":[0.9997907,0.00001355629,0.00003791651,0.0000585301,0.00007439128,0.00002490392],"domain_scores_gemma":[0.999895,0.00001170944,0.00002323877,0.00002066326,0.00001454331,0.00003485633],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003281963,0.00003230293,0.0001665708,0.00002744538,0.000005389811,0.0001595194,0.00003755383,0.00006692077,0.9979063,0.0002813944,0.0001716899,0.00111218],"study_design_scores_gemma":[0.00005129761,0.0002556641,0.004640188,0.00001632169,0.00004560937,0.00120201,0.0000669421,0.002502367,0.9719216,0.000137235,0.01913857,0.00002212802],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9349181,0.001038656,0.04616658,0.0003135565,0.0002607614,0.0005997428,0.008404947,0.0008308456,0.007466789],"genre_scores_gemma":[0.9048701,0.001673765,0.04498409,0.0002356689,0.00003967761,0.0008476141,0.01194024,0.0002730761,0.03513568],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002240424,"threshold_uncertainty_score":0.007494926,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06562680782180927,"score_gpt":0.3576540535833208,"score_spread":0.2920272457615115,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}