{"id":"W3048486047","doi":"10.1016/j.xpro.2020.100086","title":"Protocol for Primary Mouse Hepatocyte Isolation","year":2020,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Liver physiology and pathology","field":"Medicine","cited_by":415,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Azrieli Foundation; Israel Science Foundation; Abisch-Frenkel-Stiftung; International Development Research Centre","keywords":"Collagenase; Hepatocyte; Liver perfusion; Perfusion; Ex vivo; In vivo; Limiting; Protocol (science); Cell biology; Isolation (microbiology); Liver tissue; Chemistry; Biology; Medicine; Bioinformatics; Biochemistry; Pathology; In vitro; Internal medicine; Enzyme; Biotechnology; Engineering","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002043693,0.001988362,0.001495474,0.003740899,0.001265606,0.0008100858,0.001795623,0.00138048,0.03532173],"category_scores_gemma":[0.0009773877,0.0009074649,0.001186521,0.001273447,0.0006500797,0.0006241454,0.0009812743,0.004183179,0.02470433],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000607861,"about_ca_system_score_gemma":0.001222243,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006703237,"about_ca_topic_score_gemma":0.001925227,"domain_scores_codex":[0.9980129,0.0003155994,0.0002908328,0.0004312782,0.0007292577,0.000220049],"domain_scores_gemma":[0.999006,0.0001409794,0.00009231753,0.0002412192,0.0003327851,0.0001867704],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.001193243,0.0007040253,0.00077327,0.001232783,0.00009083111,0.00078768,0.0002542943,0.0005711338,0.9095006,0.004546355,0.03475269,0.04559311],"study_design_scores_gemma":[0.0003571781,0.001558636,0.004326497,0.0004041673,0.0002378497,0.002487024,0.00007192878,0.002174587,0.4323131,0.002119794,0.5537898,0.0001595058],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.05953902,0.02095276,0.7562531,0.001540201,0.004777549,0.02686469,0.04607906,0.0155195,0.06847409],"genre_scores_gemma":[0.08393385,0.01681175,0.6316669,0.003258763,0.0008991299,0.08317349,0.08352128,0.002483196,0.09425166],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.03532173,"threshold_uncertainty_score":0.118163,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09772349177661241,"score_gpt":0.3815027705552292,"score_spread":0.2837792787786169,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}