{"id":"W3079012346","doi":"10.21769/bioprotoc.3715","title":"Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay","year":2020,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre Intégré Universitaire de Santé et de Services Sociaux du Centre-Sud-de-l'Île-de-Montréal; Centre Hospitalier Universitaire de Sherbrooke; Université de Sherbrooke; McGill University","funders":"Faculty of Medicine and Health, University of Sydney; Canadian Institutes of Health Research; Réseau québécois de recherche sur la douleur; Université de Sherbrooke","keywords":"Transfection; High-throughput screening; HEK 293 cells; Mutant; Ligand binding assay; Cell culture; Receptor; G protein-coupled receptor; Chemistry; Cell biology; Molecular biology; Computational biology; Biology; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0005802722,0.0003949869,0.0003414887,0.00005141826,0.0003120197,0.0003032551,0.0001777632,0.0003410649,0.0001283619],"category_scores_gemma":[0.00005526584,0.0003378605,0.0001346239,0.0001657677,0.00007520802,0.00003614115,0.00004336896,0.0001825324,0.000003217386],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000354167,"about_ca_system_score_gemma":0.0001873151,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001961931,"about_ca_topic_score_gemma":0.000007989118,"domain_scores_codex":[0.9978015,0.0002015686,0.000439405,0.0008256859,0.0002825924,0.00044925],"domain_scores_gemma":[0.9990697,0.00005327637,0.0002441547,0.0002563331,0.0001674376,0.0002090823],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001447636,0.0002225709,0.000151253,0.0007969378,0.00004481212,0.000003868229,0.00004733487,0.0005014009,0.9928138,0.00002633825,0.0003090716,0.00363503],"study_design_scores_gemma":[0.007198876,0.003129927,0.00002997612,0.0001656372,0.00003448046,0.000003995081,0.00005815234,0.001850381,0.9281268,0.00006038729,0.0588625,0.0004789053],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.002602821,0.000001213056,0.3621178,0.0001720341,0.00002590494,0.6348549,0.0000150779,0.00005772758,0.0001524822],"genre_scores_gemma":[0.07214493,7.124507e-8,0.026404,0.0003920899,0.0004596136,0.9004174,0.00005599638,0.00008733001,0.00003850844],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.3357138,"threshold_uncertainty_score":0.9999073,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04214115711114128,"score_gpt":0.3030761444771193,"score_spread":0.260934987365978,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}