{"id":"W3081892675","doi":"10.1002/bit.27547","title":"Strain engineering for high‐level 5‐aminolevulinic acid production in <i>Escherichia coli</i>","year":2020,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Photosynthetic Processes and Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Metabolic engineering; Bioprocess; Biosynthesis; Biochemistry; Bioreactor; Strain (injury); Heterologous; Flux (metallurgy); Biology; Chemistry; Enzyme; Gene; Botany; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002852062,0.0005964163,0.0004227897,0.0002891182,0.0001622203,0.0006524589,0.0004979825,0.000401565,0.0005114387],"category_scores_gemma":[0.0002637443,0.0002279651,0.0004540088,0.0003613521,0.0002264342,0.0002149935,0.0005453128,0.00074266,0.0005603626],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003883035,"about_ca_system_score_gemma":0.0003662111,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00127146,"about_ca_topic_score_gemma":0.001363669,"domain_scores_codex":[0.9995577,0.00009246432,0.00007162144,0.00007076239,0.0001341883,0.00007324351],"domain_scores_gemma":[0.9997984,0.00003194882,0.00006381187,0.0000384711,0.00003655838,0.00003083035],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002004328,0.00003224105,0.0001387699,0.00002394017,0.000007620969,0.00004365323,0.000008589488,0.0001534949,0.9985629,0.00008468825,0.00002576835,0.0008984048],"study_design_scores_gemma":[0.000004734392,0.00007518889,0.0006673347,0.000004575098,0.00001320567,0.0001063931,0.00001932141,0.001279709,0.9967222,0.00001787609,0.001082594,0.000006942091],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9610772,0.0004344464,0.03466119,0.0002046129,0.00005850523,0.0001531185,0.0006758698,0.0004765388,0.00225848],"genre_scores_gemma":[0.9651348,0.0004462097,0.03077462,0.00008938371,0.000009969623,0.00009497621,0.001055817,0.0001794278,0.002214785],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00127146,"threshold_uncertainty_score":0.002817392,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01537094163876517,"score_gpt":0.2111464553513741,"score_spread":0.1957755137126089,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}