{"id":"W3081892675","doi":"10.1002/bit.27547","title":"Strain engineering for high‐level 5‐aminolevulinic acid production in <i>Escherichia coli</i>","year":2020,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Photosynthetic Processes and Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Metabolic engineering; Bioprocess; Biosynthesis; Biochemistry; Bioreactor; Strain (injury); Heterologous; Flux (metallurgy); Biology; Chemistry; Enzyme; Gene; Botany; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009420004,0.0001592013,0.0001572905,0.00005903011,0.00003042797,0.000009137754,0.0001264911,0.0003324054,9.874809e-7],"category_scores_gemma":[0.0001449538,0.0001657265,0.00003061612,0.0001484192,0.00003240752,0.000005297449,0.00007161887,0.0001405601,7.831816e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000768461,"about_ca_system_score_gemma":0.00002259177,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000002084034,"about_ca_topic_score_gemma":0.000004591514,"domain_scores_codex":[0.9991679,0.000004226247,0.0001653035,0.0003715441,0.00003859861,0.0002523881],"domain_scores_gemma":[0.9997533,0.000005138993,0.00003363541,0.0001316545,0.00001782188,0.0000584572],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003941635,0.00001521002,0.00001182462,0.00009682,0.00002101553,0.000001716624,0.00001763426,0.000180156,0.9967682,0.0008559555,0.00001234897,0.001979661],"study_design_scores_gemma":[0.0003843208,0.0002773918,0.0001123952,0.00002208162,0.00001163065,0.000009782971,0.00004392816,0.001968611,0.9939699,0.0000563314,0.002946112,0.0001975664],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.925406,0.0004760961,0.07108472,0.002426307,0.0001695375,0.0002826709,0.00006286253,0.00008784203,0.000003959386],"genre_scores_gemma":[0.9915981,0.0002940875,0.007638115,0.0001953737,0.0001406921,0.00006209533,0.00003508666,0.00002396242,0.00001243197],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06619214,"threshold_uncertainty_score":0.675813,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01537094163876517,"score_gpt":0.2111464553513741,"score_spread":0.1957755137126089,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}