{"id":"W3083440639","doi":"10.1007/978-1-0716-0868-5_5","title":"Study Essential Gene Functions by Plasmid Shuffling","year":2020,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Plasmid; URA3; Selectable marker; Gene; Biology; Genetics; T-DNA Binary system; Electroporation; Molecular biology; Recombinant DNA; Vector (molecular biology)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003764177,0.0007211215,0.0003829664,0.0006542282,0.0003237404,0.0004516765,0.0006112025,0.0004257273,0.002157225],"category_scores_gemma":[0.0005112645,0.0003469565,0.0005826023,0.0006433535,0.0005149679,0.0004289489,0.0007605815,0.00176459,0.0007674877],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004955275,"about_ca_system_score_gemma":0.0003736146,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006212901,"about_ca_topic_score_gemma":0.0007370703,"domain_scores_codex":[0.9997185,0.00007074254,0.00003197532,0.0000480408,0.00007443012,0.00005633942],"domain_scores_gemma":[0.9993156,0.0004346702,0.00007247924,0.00009231127,0.00002639962,0.00005858112],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001594094,0.00006906995,0.0001895847,0.0000448527,0.00001151924,0.00005465425,0.00002416591,0.0003461527,0.9956476,0.0007638464,0.00005845439,0.002630773],"study_design_scores_gemma":[0.00002934989,0.00009561737,0.0005211307,0.000002810123,0.00001562354,0.0001454473,0.00002030481,0.001643638,0.9962093,0.0002176012,0.001093505,0.00000564492],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.926709,0.001074941,0.06672934,0.0003139216,0.00008179814,0.0002413872,0.001281308,0.000537561,0.003030809],"genre_scores_gemma":[0.9731306,0.0009179757,0.02032773,0.0001054387,0.000016932,0.0001591861,0.00188491,0.0003199947,0.003137114],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002157225,"threshold_uncertainty_score":0.007216573,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03228926084457713,"score_gpt":0.4074285472317387,"score_spread":0.3751392863871615,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}