{"id":"W3083970437","doi":"10.3390/life10090186","title":"Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically Transformed Chlamydomonas reinhardtii","year":2020,"lang":"en","type":"article","venue":"Life","topic":"Algal biology and biofuel production","field":"Energy","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université du Québec à Trois-Rivières","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs","keywords":"Chlamydomonas reinhardtii; Chlamydomonas; Biology; Transformation (genetics); Computational biology; Genetically modified organism; Biotechnology; Selection (genetic algorithm); Polymerase chain reaction; Electroporation; Synthetic biology; Biochemical engineering; Gene; Genetics; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001501736,0.001101718,0.000966933,0.001252342,0.0004532388,0.0005978565,0.0009768407,0.000542715,0.001003473],"category_scores_gemma":[0.001532846,0.0006338022,0.0007593434,0.0007053824,0.0004293453,0.000463537,0.0009227968,0.001155462,0.001525463],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003442695,"about_ca_system_score_gemma":0.0007887055,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001221016,"about_ca_topic_score_gemma":0.002684343,"domain_scores_codex":[0.9977319,0.0003714107,0.0002768085,0.0004165748,0.0009927687,0.000210683],"domain_scores_gemma":[0.9992775,0.0002066925,0.00009721483,0.0001503205,0.0002094013,0.00005884631],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001703957,0.00004076837,0.0002725638,0.00007430481,0.000007452549,0.00005087921,0.00002643232,0.0001108466,0.9932811,0.0000889447,0.0002110738,0.005818486],"study_design_scores_gemma":[0.00001350606,0.0001997249,0.00347642,0.00001620776,0.00005480219,0.0002596387,0.00003482758,0.002900734,0.9871812,0.0001060123,0.005731293,0.00002557159],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2760375,0.003484953,0.7047444,0.0005019459,0.0002825272,0.001900926,0.005701248,0.004344127,0.003002393],"genre_scores_gemma":[0.3072384,0.00348224,0.6670498,0.0003014954,0.00007669746,0.002205697,0.01372862,0.0006498716,0.005267214],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001501736,"threshold_uncertainty_score":0.007942021,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03024457492596302,"score_gpt":0.2383921800333541,"score_spread":0.208147605107391,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}