{"id":"W3083970437","doi":"10.3390/life10090186","title":"Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically Transformed Chlamydomonas reinhardtii","year":2020,"lang":"en","type":"article","venue":"Life","topic":"Algal biology and biofuel production","field":"Energy","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université du Québec à Trois-Rivières","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs","keywords":"Chlamydomonas reinhardtii; Chlamydomonas; Biology; Transformation (genetics); Computational biology; Genetically modified organism; Biotechnology; Selection (genetic algorithm); Polymerase chain reaction; Electroporation; Synthetic biology; Biochemical engineering; Gene; Genetics; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001354987,0.0001068351,0.0002107353,0.00001821026,0.00008340199,0.000006521852,0.00008138905,0.0001346934,0.00008810413],"category_scores_gemma":[0.0001824282,0.00008851027,0.0000559782,0.00009308961,0.0001426853,0.00002615628,0.00002430031,0.00008452702,0.000007584645],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005186798,"about_ca_system_score_gemma":0.00002534324,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004672998,"about_ca_topic_score_gemma":0.000006783411,"domain_scores_codex":[0.9991795,0.00003976487,0.0002400137,0.0002730551,0.00008789688,0.0001797246],"domain_scores_gemma":[0.9996198,0.00007165657,0.00006074638,0.00009773632,0.00005516095,0.00009483573],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.01052448,0.0004289639,0.003343397,0.001326398,0.0009519479,0.00001339468,0.004440953,0.006053984,0.5271202,0.1407513,0.007852787,0.2971922],"study_design_scores_gemma":[0.008655454,0.005310471,0.03230491,0.00009083153,0.000399398,0.0000211423,0.0005552224,0.01335622,0.6573926,0.00372201,0.277111,0.001080804],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.970336,0.001302789,0.01529183,0.01093401,0.000231097,0.0004546727,0.00003447276,0.00008346258,0.00133167],"genre_scores_gemma":[0.9932712,0.00009889649,0.004934534,0.001192787,0.0003705986,0.00001969078,0.00003908693,0.0000120495,0.00006115043],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2961113,"threshold_uncertainty_score":0.3609344,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03024457492596302,"score_gpt":0.2383921800333541,"score_spread":0.208147605107391,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}