{"id":"W3084025640","doi":"10.1101/2020.09.08.286732","title":"Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"SARS-CoV-2 and COVID-19 Research","field":"Medicine","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Ectodomain; Chinese hamster ovary cell; Spike (software development); Recombinant DNA; Transfection; HEK 293 cells; Gene; Yield (engineering); Cell culture; Cell biology; Biology; Molecular biology; Chemistry; Computational biology; Biochemistry; Genetics; Computer science; Materials science; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006674818,0.00055956,0.0007271923,0.0003808405,0.0003038954,0.0007595399,0.0005529399,0.0004875039,0.001234767],"category_scores_gemma":[0.0003491444,0.0002668702,0.0005827425,0.0005865032,0.0002900983,0.000417163,0.0005601713,0.0009941497,0.001782604],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004846719,"about_ca_system_score_gemma":0.0003333846,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006246322,"about_ca_topic_score_gemma":0.0007240328,"domain_scores_codex":[0.9995704,0.00006372673,0.00006776059,0.00007359734,0.000171619,0.00005288752],"domain_scores_gemma":[0.9998026,0.00004457088,0.00002815612,0.00004772764,0.00005694763,0.00002000133],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002939856,0.00001788781,0.00003706819,0.0000419857,0.000004869039,0.00002809495,0.0000185891,0.00004248191,0.9988227,0.00009070987,0.00007535275,0.0007908253],"study_design_scores_gemma":[0.00002755488,0.0001800447,0.0005683035,0.000006992846,0.00001887595,0.0001238217,0.00002359206,0.0009261203,0.9935983,0.00005440176,0.004462671,0.000009332891],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.761852,0.003289687,0.2106207,0.0005171902,0.0003480527,0.001512928,0.008556835,0.002133758,0.01116886],"genre_scores_gemma":[0.8073104,0.003412837,0.1563946,0.0002395373,0.00008344405,0.001245387,0.01588531,0.0004890906,0.01493947],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001234767,"threshold_uncertainty_score":0.004130721,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03299168974085862,"score_gpt":0.2714392449985276,"score_spread":0.238447555257669,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}