{"id":"W3086085153","doi":"10.1016/j.xpro.2020.100104","title":"Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence","year":2020,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Canadian Institutes of Health Research; Université de Montréal","keywords":"RNA; Telomere; Immunofluorescence; Cajal body; Fluorescence in situ hybridization; Telomerase; Fish <Actinopterygii>; In situ hybridization; Nucleoplasm; Biology; Molecular biology; Cell biology; Nucleus; Messenger RNA; Genetics; DNA; Gene; Antibody","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006508584,0.0006258395,0.0004682451,0.0006983252,0.0003910139,0.0006226544,0.0007439779,0.0007174987,0.005002643],"category_scores_gemma":[0.0004087863,0.0005808859,0.000487213,0.0003481834,0.0004432113,0.0006434449,0.0005969023,0.001029535,0.002495086],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007032597,"about_ca_system_score_gemma":0.0005034339,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001799668,"about_ca_topic_score_gemma":0.004010619,"domain_scores_codex":[0.999616,0.00002981768,0.00001826641,0.0001281781,0.0001448449,0.00006281057],"domain_scores_gemma":[0.999782,0.00006585274,0.00002565224,0.00004816286,0.00004868479,0.00002968722],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001313201,0.000005146338,0.00005703748,0.00003405421,0.000002723403,0.00001822733,0.00001075862,0.00008359456,0.9976472,0.0002388595,0.0001032245,0.001786068],"study_design_scores_gemma":[0.00001507121,0.00007227177,0.001434323,0.00001540221,0.00001580538,0.0001840171,0.00002041603,0.003836125,0.9865888,0.0002502379,0.0075541,0.00001352723],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3892169,0.00280397,0.5794486,0.000487555,0.0003096928,0.0006211526,0.002739252,0.003667573,0.02070531],"genre_scores_gemma":[0.3794447,0.003619863,0.5770966,0.0003369294,0.00009516581,0.001389964,0.002701737,0.001043192,0.03427195],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005002643,"threshold_uncertainty_score":0.01673549,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01153427727796481,"score_gpt":0.2703479902296974,"score_spread":0.2588137129517326,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}