{"id":"W3088244240","doi":"10.1038/s41467-020-18667-3","title":"Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance","year":2020,"lang":"en","type":"article","venue":"Nature Communications","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Agencia Estatal de Investigación; Ministerio de Economía y Competitividad; Ministerio de Ciencia, Innovación y Universidades; Comunidad de Madrid","keywords":"Biocatalysis; Glycoconjugate; Acceptor; Chemistry; Glycosylation; Protein engineering; Glycoside hydrolase; Biochemistry; Enzyme; Mutant; Stereochemistry; Combinatorial chemistry; Catalysis; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.00005249225,0.0003700984,0.0003863994,0.00002840338,0.0004317123,0.0001292826,0.001920649,0.0005468701,0.001656681],"category_scores_gemma":[0.0003094844,0.0003497835,0.0001887908,0.0003904459,0.0002418937,0.0002051944,0.0004671745,0.001690796,0.0001043176],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008721436,"about_ca_system_score_gemma":0.0001477103,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001175156,"about_ca_topic_score_gemma":0.0002036168,"domain_scores_codex":[0.998377,0.0000601723,0.0003674881,0.0005549927,0.0003019021,0.0003384069],"domain_scores_gemma":[0.9961368,0.0004172357,0.000208979,0.002634121,0.0002104084,0.0003924194],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003123713,0.0004092177,0.003609904,0.0000734807,0.0002106059,0.00003990445,0.002903176,0.00002105472,0.9859271,0.00005106753,0.005407588,0.001034496],"study_design_scores_gemma":[0.001566239,0.00001661639,0.0009262734,0.0001237894,0.0001693248,0.00001260864,0.0007624919,0.004815319,0.9503224,0.00001473487,0.04071962,0.0005505924],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9686507,0.006092814,0.00009095224,0.01396918,0.00005084403,0.000229066,0.001582057,0.0004195129,0.008914875],"genre_scores_gemma":[0.9856192,0.0003335951,0.008883348,0.003966229,0.0002373631,0.0001588737,0.0004377848,0.00005967882,0.0003038699],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03560475,"threshold_uncertainty_score":0.9998954,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02872575328916365,"score_gpt":0.2705065057982348,"score_spread":0.2417807525090711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}