{"id":"W3088504822","doi":"10.1074/jbc.ra120.015360","title":"Development and validation of a high-throughput whole cell assay to investigate Staphylococcus aureus adhesion to host ligands","year":2020,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Social Sciences and Humanities Research Council of Canada; Government of Canada; Canada Foundation for Innovation; J.P. Bickell Foundation","keywords":"Sortase; Bacterial adhesin; Cell adhesion; Sortase A; Adhesion; Biology; Staphylococcus aureus; Mutant; High-throughput screening; Fibronectin; Cell adhesion molecule; Cell biology; Microbiology; Computational biology; Genetics; Gene; Cell; Chemistry; Extracellular matrix; Escherichia coli; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001366779,0.0001432531,0.0002332209,0.00001395936,0.00003188595,0.00001677719,0.0001657151,0.0002080748,0.00001323511],"category_scores_gemma":[0.0002680564,0.0001023686,0.00005614389,0.0001131142,0.00004320843,0.000005597034,0.000152481,0.0001195482,0.000002183986],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001358087,"about_ca_system_score_gemma":0.00005234792,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":8.047801e-7,"about_ca_topic_score_gemma":7.889905e-8,"domain_scores_codex":[0.9990662,0.000019901,0.0004126194,0.0002273604,0.0001362069,0.0001376935],"domain_scores_gemma":[0.9992064,0.00001199077,0.0002312855,0.00008482861,0.0001503309,0.0003151974],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002123997,0.00002493125,0.0009555703,0.00005310836,0.00001601531,0.000002801532,0.000100631,0.00001102148,0.9977363,9.416005e-7,0.0003546402,0.0005316429],"study_design_scores_gemma":[0.0003004302,0.0004159304,0.004344555,0.00002865724,0.000008750964,0.000005413307,0.0000465624,0.000002257456,0.9889814,0.00001720636,0.005707711,0.0001410818],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979039,0.0001200878,0.0005631901,0.001244583,0.00003387605,0.00007682282,0.00002409758,0.000004501539,0.00002892329],"genre_scores_gemma":[0.9935987,0.00003304835,0.005300192,0.0006884611,0.0002628851,0.000002470948,0.00008060692,0.00000689102,0.00002675139],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008754852,"threshold_uncertainty_score":0.4174469,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01914097496450557,"score_gpt":0.2270426932793438,"score_spread":0.2079017183148383,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}