{"id":"W30886713","doi":"10.1096/fasebj.20.5.lb54-b","title":"Expression and purification of active protein kinase Cd (PKCd) kinase domain from E. coli","year":2006,"lang":"en","type":"article","venue":"The FASEB Journal","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Women's Health Research Institute","funders":"","keywords":"Protein kinase C; Protein kinase domain; Kinase; Recombinant DNA; Enzyme; Isozyme; Biochemistry; Molecular biology; Chemistry; Cyclin-dependent kinase 9; Biology; Mitogen-activated protein kinase kinase; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00026208,0.0001098109,0.0001068655,0.00003810658,0.0001850322,0.00003355352,0.0001199151,0.00008901004,0.00004136134],"category_scores_gemma":[0.00003638012,0.00007857813,0.00006163641,0.00005616091,0.0001072616,0.00001068762,0.00004809029,0.0001250709,0.000001908895],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001396514,"about_ca_system_score_gemma":0.00005079115,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005652348,"about_ca_topic_score_gemma":0.00001288844,"domain_scores_codex":[0.9991536,0.0001662097,0.000228501,0.0001554071,0.000162488,0.0001338184],"domain_scores_gemma":[0.9993898,0.00001535095,0.0002425585,0.0002029248,0.00008680622,0.00006257997],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002386493,0.00004952733,0.00008739499,0.000005415414,0.00001268913,0.000005698233,0.00007279482,0.00005445158,0.9958473,0.0002672128,0.0001333452,0.003225506],"study_design_scores_gemma":[0.0007356742,0.00006214031,0.003051647,0.00006822835,0.00001413935,0.00001901134,0.0001757743,0.00003526562,0.9898027,0.004693819,0.001243911,0.00009774159],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9918423,0.001697576,0.005770729,0.000200044,0.00003801956,0.0002255801,0.00004495268,0.000005062111,0.0001757197],"genre_scores_gemma":[0.997188,0.00004449512,0.002197169,0.00003560194,0.0003454873,0.0000141285,0.00007216461,0.00001194098,0.00009104476],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006044666,"threshold_uncertainty_score":0.3204323,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009072900708121232,"score_gpt":0.2264780253877245,"score_spread":0.2174051246796033,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}