{"id":"W3092009066","doi":"10.1021/acs.biochem.0c00568","title":"A Key Glycine in Bacterial Steroid-Degrading Acyl-CoA Dehydrogenases Allows Flavin-Ring Repositioning and Modulates Substrate Side Chain Specificity","year":2020,"lang":"en","type":"article","venue":"Biochemistry","topic":"Steroid Chemistry and Biochemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Side chain; Flavin group; Glycine; Chemistry; Ring (chemistry); Biochemistry; Substrate specificity; Steroid; Substrate (aquarium); Stereochemistry; Flavoprotein; Combinatorial chemistry; Enzyme; Biology; Amino acid; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001724285,0.0004555955,0.0004018038,0.0001400914,0.0002658546,0.000316259,0.0004175078,0.0005063127,0.001551195],"category_scores_gemma":[0.000317961,0.0001865478,0.0002746261,0.0001680593,0.0003690502,0.0003518409,0.0003888286,0.0005230411,0.001356289],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003114295,"about_ca_system_score_gemma":0.0002587662,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003523833,"about_ca_topic_score_gemma":0.0003577933,"domain_scores_codex":[0.9998212,0.00002752601,0.0000159891,0.00006794592,0.00004230014,0.0000249818],"domain_scores_gemma":[0.9998146,0.00003041344,0.0000588758,0.00003288741,0.00001574651,0.00004752935],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002172502,0.00001938711,0.0005636927,0.00005175309,0.000006460939,0.0002366325,0.00003101564,0.0001748037,0.9939026,0.0003544847,0.00006527974,0.004376614],"study_design_scores_gemma":[0.00004081244,0.0003757429,0.005839468,0.00001307185,0.00001696876,0.001125475,0.00004160371,0.001041118,0.9831088,0.0002964917,0.008081377,0.00001904684],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9877487,0.001347914,0.007797499,0.0002108833,0.00007934443,0.00003812175,0.0003150323,0.0004326223,0.002029891],"genre_scores_gemma":[0.9919869,0.0002628825,0.005848381,0.00007955868,0.00001415436,0.00001942896,0.0002440386,0.00006459143,0.001480074],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001551195,"threshold_uncertainty_score":0.00518924,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01351197078227932,"score_gpt":0.2193083293987571,"score_spread":0.2057963586164778,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}